Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus amyloliquefaciens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 10 mM, complete loss of activity | Bacillus amyloliquefaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.62 | - |
D-mannose 6-phosphate | pH 7.5, 70°C | Bacillus amyloliquefaciens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | most effective activator of EDTA-inactivated enzyme, 1.7fold activation at 0.5 mM compared to initial activity | Bacillus amyloliquefaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
gel filtration | Bacillus amyloliquefaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus amyloliquefaciens | - |
- |
- |
Bacillus amyloliquefaciens DSM 7 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
81 | - |
substrate D-mannose 6-phosphate, pH 7.5, 70°C | Bacillus amyloliquefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Mannose 6-phosphate | - |
Bacillus amyloliquefaciens | D-Fructose 6-phosphate | - |
? | |
D-Mannose 6-phosphate | - |
Bacillus amyloliquefaciens DSM 7 | D-Fructose 6-phosphate | - |
? | |
D-ribose | poor substrate | Bacillus amyloliquefaciens | D-ribulose | - |
? | |
D-ribose | poor substrate | Bacillus amyloliquefaciens DSM 7 | D-ribulose | - |
? | |
L-ribulose | poor substrate | Bacillus amyloliquefaciens | L-ribose | - |
? | |
L-ribulose | poor substrate | Bacillus amyloliquefaciens DSM 7 | L-ribose | - |
? | |
L-talose | poor substrate | Bacillus amyloliquefaciens | L-tagatose | - |
? | |
L-talose | poor substrate | Bacillus amyloliquefaciens DSM 7 | L-tagatose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 35490, calculated from sequence, 1 * 35000, SDS-PAGE | Bacillus amyloliquefaciens |
Synonyms | Comment | Organism |
---|---|---|
M6PI | - |
Bacillus amyloliquefaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Bacillus amyloliquefaciens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
presence of Co2+, half-life 15 h | Bacillus amyloliquefaciens |
50 | - |
presence of Co2+, half-life 3.5 h | Bacillus amyloliquefaciens |
60 | - |
presence of Co2+, half-life 10 min | Bacillus amyloliquefaciens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
106000 | - |
D-mannose 6-phosphate | pH 7.5, 70°C | Bacillus amyloliquefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Bacillus amyloliquefaciens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | - |
83% of maximum activity | Bacillus amyloliquefaciens |
9 | - |
94% of maximum activity | Bacillus amyloliquefaciens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13900 | - |
D-mannose 6-phosphate | pH 7.5, 70°C | Bacillus amyloliquefaciens |