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Literature summary for 5.3.1.8 extracted from

  • Sousa, S.A.; Moreira, L.M.; Wopperer, J.; Eberl, L.; Sa-Correira, I.; Leitao, J.H.
    The Burkholderia cepacia bceA gene encodes a protein with phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activities (2007), Biochem. Biophys. Res. Commun., 353, 200-206 .
    View publication on PubMed
Search for more literature for 5.3.1.8

Cloned(Commentary)

Cloned (Comment) Organism
expression of the His-tagged enzyme in Escherichia coli Burkholderia cepacia

Inhibitors

Inhibitors Comment Organism Structure
EDTA no activity is detected when 2 mM EDTA is added to the dialysis buffer Burkholderia cepacia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
9.01
-
 D-mannose 6-phosphate pH 7.6, 5 mM Mg2+, temperature not specified in the publication Burkholderia cepacia

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ divalent ions are necessary for PMI maximal activity in decreasing order of activation: Ca2+, Mn2+, Mg2+, Co2+, Ni2+ Burkholderia cepacia
Co2+ divalent ions are necessary for PMI maximal activity in decreasing order of activation: Ca2+, Mn2+, Mg2+, Co2+, Ni2+ Burkholderia cepacia
Mg2+ divalent ions are necessary for PMI maximal activity in decreasing order of activation: Ca2+, Mn2+, Mg2+, Co2+, Ni2+ Burkholderia cepacia
Mn2+ divalent ions are necessary for PMI maximal activity in decreasing order of activation: Ca2+, Mn2+, Mg2+, Co2+, Ni2+ Burkholderia cepacia
Ni2+ divalent ions are necessary for PMI maximal activity in decreasing order of activation: Ca2+, Mn2+, Mg2+, Co2+, Ni2+ Burkholderia cepacia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 SDS-PAGE Burkholderia cepacia
55300
-
 calculated from sequence Burkholderia cepacia

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia Q06XM8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Burkholderia cepacia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Mannose 6-phosphate
-
 Burkholderia cepacia D-Fructose 6-phosphate
-
 ?
D-Mannose 6-phosphate
-
 Burkholderia cepacia IST408 D-Fructose 6-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
BceA
-
 Burkholderia cepacia
Phosphomannose isomerase
-
 Burkholderia cepacia
PMI
-
 Burkholderia cepacia

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
19.4
-
 D-mannose 6-phosphate pH 7.6, 5 mM Mg2+, temperature not specified in the publication Burkholderia cepacia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Burkholderia cepacia

pI Value

Organism Comment pI Value Maximum pI Value
Burkholderia cepacia calculated from sequence
-
 6.03

General Information

General Information Comment Organism
malfunction the lack of a functional bceA gene does not affect exopolysaccharide production yield in a non-polar insertion bceA mutant. The in silico search for putative bceA homologues reveals the presence of 2-5 bceA orthologues in the Burkholderia genomes available. This suggests that in Burkholderia cepacia IST408 putative bceA functional homologues may compensate the bceA mutation Burkholderia cepacia
physiological function the enzyme is involved in cepacian production yield and rheological properties and in the size of biofilms formed Burkholderia cepacia