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Literature summary for 5.3.1.8 extracted from

  • Yeom, S.J.; Kim, N.H.; Park, C.S.; Oh, D.K.
    L-ribose production from L-arabinose by using purified L-arabinose isomerase and mannose-6-phosphate isomerase from Geobacillus thermodenitrificans (2009), Appl. Environ. Microbiol., 75, 6941-6943.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis L-ribose production of the enzyme in a coupled assay system with L-arabinose isomerase, EC 5.3.1.4, in recombinant Escherichia coli ER2566, AI/MPI ratio, 1:2.5, method optimization. L-Ribose is a potential starting material for the synthesis of many L-nucleoside-based pharmaceutical compounds Geobacillus thermodenitrificans

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain ER2566, coexpression with L-arabinose isomerase Geobacillus thermodenitrificans

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+
-
Geobacillus thermodenitrificans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-Mannose 6-phosphate Geobacillus thermodenitrificans
-
D-Fructose 6-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Geobacillus thermodenitrificans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Mannose 6-phosphate
-
Geobacillus thermodenitrificans D-Fructose 6-phosphate
-
r

Synonyms

Synonyms Comment Organism
MPI
-
Geobacillus thermodenitrificans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
assay at Geobacillus thermodenitrificans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Geobacillus thermodenitrificans