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Literature summary for 5.3.1.8 extracted from
- Substrate specificity of a mannose-6-phosphate isomerase from Bacillus subtilis and its application in the production of L-ribose (2009), Appl. Environ. Microbiol., 75, 4705-4710.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | mannose-6-phosphate isomerase from Bacillus subtilis is applied for the production of L-ribose by direct isomerization of L-ribulose | Bacillus subtilis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli strain ER2566 | Bacillus subtilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | complete inactivation | Bacillus subtilis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 110 | - |
D-ribose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
| 312 | - |
L-allose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
| 433 | - |
D-Lyxose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
| 469 | - |
D-talose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
| 946 | - |
D-mannose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
| 998 | - |
L-ribose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | activates | Bacillus subtilis | |
| Ca2+ | activates | Bacillus subtilis | |
| Co2+ | activates, best metal ion, maximal activity at 0.5 mM | Bacillus subtilis | |
| Cu2+ | activates | Bacillus subtilis | |
| Mg2+ | activates | Bacillus subtilis | |
| Mn2+ | highly activating | Bacillus subtilis | |
| additional information | mannose-6-phosphate isomerases are metalloenzymes that require a divalent ion metal cofactor for activity and catalysis | Bacillus subtilis | |
| Ni2+ | activates | Bacillus subtilis | |
| Zn2+ | activates | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36444 | - |
* 36500, recombinant enzyme, SDS-PAGE, 1 * 36444, sequence calculation | Bacillus subtilis |
| 36500 | - |
* 36500, recombinant enzyme, SDS-PAGE, 1 * 36444, sequence calculation | Bacillus subtilis |
| 36600 | - |
gel filtration, recombinant enzyme | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Mannose 6-phosphate | Bacillus subtilis | - |
D-Fructose 6-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | Q9AGZ4 | enzyme from Bacillus subtilis strain HB002; ATCC 23857 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme 27fold from Escherichia coli strain ER2566 by affinity chromatography | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
substrate specificity, overview | Bacillus subtilis |
| 0.0101 | - |
recombinant enzyme | Bacillus subtilis |
| 0.0165 | - |
recombinant enzyme | Bacillus subtilis |
| 0.0225 | - |
recombinant enzyme | Bacillus subtilis |
| 0.0659 | - |
recombinant enzyme | Bacillus subtilis |
| 0.0918 | - |
recombinant enzyme | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-allose | - |
Bacillus subtilis | D-psicose | - |
r | |
| D-Lyxose | - |
Bacillus subtilis | D-Xylulose | - |
r | |
| D-Mannose | - |
Bacillus subtilis | D-Fructose | - |
r | |
| D-Mannose 6-phosphate | - |
Bacillus subtilis | D-Fructose 6-phosphate | - |
r | |
| D-ribose | - |
Bacillus subtilis | D-ribulose | - |
r | |
| D-talose | - |
Bacillus subtilis | D-tagatose | - |
r | |
| L-allose | - |
Bacillus subtilis | L-psicose | - |
r | |
| L-lyxose | - |
Bacillus subtilis | L-xylulose | - |
r | |
| L-Mannose | - |
Bacillus subtilis | L-Fructose | - |
r | |
| L-ribose | - |
Bacillus subtilis | L-ribulose | - |
r | |
| L-ribulose | best substrate | Bacillus subtilis | L-ribose | - |
r | |
| L-talose | - |
Bacillus subtilis | L-tagatose | - |
r | |
| additional information | the enzyme is specific for aldose substrates possessing hydroxyl groups oriented in the same direction at the C-2 and C-3 positions, such as the D- and L-enantiomers of ribose, lyxose, talose, mannose, and allose, substrate specificity, overview | Bacillus subtilis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | * 36500, recombinant enzyme, SDS-PAGE, 1 * 36444, sequence calculation | Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
recombinant enzyme | Bacillus subtilis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | - |
Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
first-order kinetics for thermal inactivation | Bacillus subtilis |
| 25 | - |
purified recombinant enzyme, half-life is 461 h | Bacillus subtilis |
| 30 | - |
purified recombinant enzyme, half-life is 325 h | Bacillus subtilis |
| 35 | - |
purified recombinant enzyme, half-life is 236 h | Bacillus subtilis |
| 40 | - |
purified recombinant enzyme, half-life is 111 h | Bacillus subtilis |
| 45 | - |
purified recombinant enzyme, half-life is 56 h | Bacillus subtilis |
| 50 | - |
purified recombinant enzyme, half-life is 10 h | Bacillus subtilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 72 | - |
D-ribose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
| 920 | - |
L-allose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
| 3748 | - |
D-mannose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
| 7089 | - |
D-Lyxose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
| 17600 | - |
L-ribose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
| 63970 | - |
D-talose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
recombinant enzyme | Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | - |
Bacillus subtilis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
D-ribose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
| 2.9 | - |
L-allose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
| 3.5 | - |
D-mannose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
| 6.8 | - |
D-talose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
| 16.4 | - |
D-Lyxose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
| 17.6 | - |
L-ribose | pH 7.5, 40°C, recombinant enzyme | Bacillus subtilis | |
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