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Literature summary for 5.3.1.8 extracted from
- Bifunctional phosphoglucose/phosphomannose isomerase from the hyperthermophilic archaeon Pyrobaculum aerophilum (2004), Extremophiles, 8, 507-512.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Pyrobaculum aerophilum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 6-phosphogluconate | - |
Pyrobaculum aerophilum |  |
| erythrose 4-phosphate | - |
Pyrobaculum aerophilum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
D-fructose 6-phosphate | pH 7.4, 80°C | Pyrobaculum aerophilum | |
| 0.06 | - |
D-mannose 6-phosphate | pH 7.4, 80°C | Pyrobaculum aerophilum | |
| 0.3 | - |
D-fructose 6-phosphate | pH 7.4, 50°C | Pyrobaculum aerophilum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33548 | - |
2 * 36000, SDS-PAGE, 2 * 33548, calculated | Pyrobaculum aerophilum |
| 36000 | - |
2 * 36000, SDS-PAGE, 2 * 33548, calculated | Pyrobaculum aerophilum |
| 65000 | - |
sedimentation equilibrium, analytical ultracentrifugation | Pyrobaculum aerophilum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrobaculum aerophilum | - |
bifunctional phosphoglucose/phosphomannose isomerase EC 5.3.1.8 and EC 5.3.1.9, overexpression in Escherichia coli | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 6-phosphate | - |
Pyrobaculum aerophilum | D-mannose 6-phosphate | - |
r | |
| D-Mannose 6-phosphate | - |
Pyrobaculum aerophilum | D-Fructose 6-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 36000, SDS-PAGE, 2 * 33548, calculated | Pyrobaculum aerophilum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
- |
Pyrobaculum aerophilum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
melting temperature for thermal unfolding, 60 min, 50% residual activity | Pyrobaculum aerophilum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Pyrobaculum aerophilum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | 8.8 | more than 50% of maximum activity in this range | Pyrobaculum aerophilum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0014 | - |
erythrose-4-phosphate | pH 7.4, 50°C | Pyrobaculum aerophilum | |
| 0.11 | - |
6-phosphogluconate | pH 7.4, 50°C | Pyrobaculum aerophilum | |
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Release 2023.1 (January 2023)
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