Literature summary for 5.3.1.8 extracted from

Shinabarger, D.; Berry, A.; May, T.B.; Rothmel, R.; Fialho, A.; Chakrabarty, A.M.
Purification and characterization of phosphomannose isomerase-guanosine diphospho-D-mannose pyrophosphorylase. A bifunctional enzyme in the alginate biosynthetic pathway of Pseudomonas aeruginosa (1991), J. Biol. Chem., 266, 2080-2088.

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Pseudomonas aeruginosa

Protein Variants

Protein Variants	Comment	Organism
additional information	point mutation at nucleotide 961 greatly decreases enzyme activity	Pseudomonas aeruginosa

Inhibitors

Inhibitors	Comment	Organism	Structure
dithiothreitol	-	Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.03	-	mannose 6-phosphate	bifunctional enzyme: phosphomannose isomerase-guanosine 5'-diphospho-D-mannose pyrophosphorylase	Pseudomonas aeruginosa

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	activates	Pseudomonas aeruginosa
Co2+	best activator	Pseudomonas aeruginosa
Mg2+	activates	Pseudomonas aeruginosa
Mn2+	activates	Pseudomonas aeruginosa
additional information	activated by bivalent cations in the order: Co2+, Ni2+, Mn2+, Mg2+, Ca2+, Zn2+	Pseudomonas aeruginosa
Ni2+	activates	Pseudomonas aeruginosa
Zinc	weak activation	Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
54000	-	bifunctional enzyme: phosphomannose isomerase-guanosine 5'-diphospho-D-mannose pyrophosphorylase, gel filtration	Pseudomonas aeruginosa
56000	-	1 * 56000, bifunctional enzyme: phosphomannose isomerase-guanosine 5-diphospho-D-mannose pyrophosphorylase, SDS-PAGE	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	-	bifunctional enzyme: phosphomannose isomerase-guanosine 5'-diphospho-D-mannose pyrophosphorylase	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-Mannose 6-phosphate	-	Pseudomonas aeruginosa	D-Fructose 6-phosphate	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 56000, bifunctional enzyme: phosphomannose isomerase-guanosine 5'-diphospho-D-mannose pyrophosphorylase, SDS-PAGE	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)