Literature summary for 5.3.1.7 extracted from

Yu, L.; Zhang, W.; Zhang, T.; Jiang, B.; Mu, W.
Efficient biotransformation of D-fructose to D-mannose by a thermostable D-lyxose isomerase from Thermosediminibacter oceani (2016), Process Biochem., 51, 2026-2033 .

No PubMed abstract available

Search for more literature for 5.3.1.7

Application

Application	Comment	Organism
synthesis	under optimum conditions, 101.6 g/l D-mannose is produced from 400 g/l D-fructose after reaction for 9 h, giving a conversion yield of 25.4%	Thermosediminibacter oceani

Inhibitors

Inhibitors	Comment	Organism	Structure
Ba2+	1 mM, 64% residual activity	Thermosediminibacter oceani
Cu2+	1 mM, 16% residual activity	Thermosediminibacter oceani
Zn2+	1 mM, 13% residual activity	Thermosediminibacter oceani

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
15.5	-	D-Lyxose	pH 6.5, 65°C	Thermosediminibacter oceani
27.3	-	D-fructose	pH 6.5, 65°C	Thermosediminibacter oceani
32.8	-	D-mannose	pH 6.5, 65°C	Thermosediminibacter oceani

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	1 mM, 9.1fold activation	Thermosediminibacter oceani
Fe2+	1 mM, 2.1fold activation	Thermosediminibacter oceani
Mn2+	1 mM, 12.5fold activation	Thermosediminibacter oceani
Ni2+	1 mM, 11.8fold activation	Thermosediminibacter oceani

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
44000	-	gel filtration	Thermosediminibacter oceani

Organism

Organism	UniProt	Comment	Textmining
Thermosediminibacter oceani	D9RZ53	cf. EC 5.3.1.15	-
Thermosediminibacter oceani DSM 16646	D9RZ53	cf. EC 5.3.1.15	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose	-	Thermosediminibacter oceani	D-mannose	-	r
D-fructose	-	Thermosediminibacter oceani DSM 16646	D-mannose	-	r
D-fructose	-	Thermosediminibacter oceani DSM 1664	D-mannose	-	r
D-Lyxose	reaction of EC 5.3.1.15	Thermosediminibacter oceani	D-Xylulose	-	r
D-Lyxose	reaction of EC 5.3.1.15	Thermosediminibacter oceani DSM 16646	D-Xylulose	-	r
D-Lyxose	reaction of EC 5.3.1.15	Thermosediminibacter oceani DSM 1664	D-Xylulose	-	r

Subunits

Subunits	Comment	Organism
dimer	2 * 22000, SDS-PAGE	Thermosediminibacter oceani

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
65	-	-	Thermosediminibacter oceani

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
70	-	half-life 5.64 h	Thermosediminibacter oceani
80	-	half-life 0.77 h	Thermosediminibacter oceani
85	-	half-life 0.2 h	Thermosediminibacter oceani

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
17.2	-	D-fructose	pH 6.5, 65°C	Thermosediminibacter oceani
51.8	-	D-Lyxose	pH 6.5, 65°C	Thermosediminibacter oceani
94.8	-	D-mannose	pH 6.5, 65°C	Thermosediminibacter oceani

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	-	Thermosediminibacter oceani

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	8	more than 50% of maximum activity	Thermosediminibacter oceani

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.63	-	D-fructose	pH 6.5, 65°C	Thermosediminibacter oceani
2.88	-	D-mannose	pH 6.5, 65°C	Thermosediminibacter oceani
3.35	-	D-Lyxose	pH 6.5, 65°C	Thermosediminibacter oceani

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Release 2023.1 (January 2023)