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Literature summary for 5.3.1.7 extracted from
- Development of a recombinant D-mannose isomerase and its characterizations for D-mannose synthesis (2016), Int. J. Biol. Macromol., 89, 328-335 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | expression in Bacillus subtilis leads to secretion of enzyme. Under optimum conditions, production of D-mannose using crude enzyme reaches about 150 g/l with approximately 25% turnover yield | Escherichia coli |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Bacillus subtilis | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 86.4 | - |
D-mannose | pH 7.0, 45°C | Escherichia coli |  |
| 203.7 | - |
D-fructose | pH 7.0, 45°C | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 274500 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | AJH12524 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose | - |
Escherichia coli | D-mannose | - |
r | |
| D-Mannose | - |
Escherichia coli | D-Fructose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 45000, calculated from sequence, 6 * 45800, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| yihS | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 61.4 | - |
melting temperature | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 27.7 | - |
D-fructose | pH 7.0, 45°C | Escherichia coli | |
| 40.9 | - |
D-mannose | pH 7.0, 45°C | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Escherichia coli |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.136 | - |
D-fructose | pH 7.0, 45°C | Escherichia coli | |
| 0.473 | - |
D-mannose | pH 7.0, 45°C | Escherichia coli | |
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