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Literature summary for 5.3.1.7 extracted from

  • Hirose, J.; Maeda, K.; Yokoi, H.; Takasaki, Y.
    Purification and characterization of mannose isomerase from Agrobacterium radiobacter M-1 (2001), Biosci. Biotechnol. Biochem., 65, 658-661.
    View publication on PubMed
Search for more literature for 5.3.1.7

General Stability

General Stability Organism
no loss of activity after 24 h dialysis against 50 mM Tris-HCl buffer containing 1 mM EDTA Agrobacterium tumefaciens

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 2 mM, complete loss of activity Agrobacterium tumefaciens
Co2+ 2 mM, partial loss of activity Agrobacterium tumefaciens
Cu2+ 2 mM, complete loss of activity Agrobacterium tumefaciens
D-mannitol 50 mM, slight inhibition Agrobacterium tumefaciens
Fe2+ 2 mM, complete loss of activity Agrobacterium tumefaciens
Fe3+ 2 mM, complete loss of activity Agrobacterium tumefaciens
Hg2+ 2 mM, complete loss of activity Agrobacterium tumefaciens
N-bromosuccinimide partial inhibition at 0.1 mM, complete inhibition at 1 mM Agrobacterium tumefaciens
Ni2+ 2 mM, partial loss of activity Agrobacterium tumefaciens
PCMB partial inhibition at 0.1 mM, complete inhibition at 1 mM Agrobacterium tumefaciens
phenylmethylsulfonyl fluoride partial inhibition at 0.1 mM, complete inhibition at 1 mM Agrobacterium tumefaciens
Zn2+ 2 mM, complete loss of activity Agrobacterium tumefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
20
-
 D-mannose pH 7.5, 35°C Agrobacterium tumefaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 2 * 44000, SDS-PAGE Agrobacterium tumefaciens
90000
-
 gel filtration Agrobacterium tumefaciens

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
-
-
Agrobacterium tumefaciens M-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Agrobacterium tumefaciens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
231
-
-
 Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Lyxose
-
 Agrobacterium tumefaciens ?
-
 ?
D-Lyxose
-
 Agrobacterium tumefaciens M-1 ?
-
 ?
D-Mannose
-
 Agrobacterium tumefaciens D-Fructose
-
 ?
D-Mannose
-
 Agrobacterium tumefaciens M-1 D-Fructose
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 44000, SDS-PAGE Agrobacterium tumefaciens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Agrobacterium tumefaciens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 65 35°C: about 55% of maximal activity, 65°C: about 10% of maximal activity, 70°C: about 20% of maximal activity Agrobacterium tumefaciens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 pH 7.5, 10 min, stable below Agrobacterium tumefaciens
55
-
 pH 7.5, 10 min, about 10% loss of activity Agrobacterium tumefaciens
60
-
 pH 7.5, 10 min, about 40% loss of activity Agrobacterium tumefaciens
70
-
 pH 7.5, 10 min, complete loss of activity Agrobacterium tumefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Agrobacterium tumefaciens

pH Range

pH Minimum pH Maximum Comment Organism
6 10.5 pH 6.0: about 65% of maximal activity, pH 10.5: about 50% of maximal activity Agrobacterium tumefaciens

pI Value

Organism Comment pI Value Maximum pI Value
Agrobacterium tumefaciens isoelectric focusing
-
 5.2