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Literature summary for 5.3.1.6 extracted from

  • Kaur, P.; Tripathi, N.; Desale, J.; Neelagiri, S.; Yadav, S.; Bharatam, P.; Singh, S.
    Mutational and structural analysis of conserved residues in ribose-5-phosphate isomerase B from Leishmania donovani Role in substrate recognition and conformational stability (2016), PLoS ONE, 11, e0150764 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Leishmania donovani

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling of wild type and mutants Leishmania donovani

Protein Variants

Protein Variants Comment Organism
C69S complete loss of enzyme activity, altered protein structure Leishmania donovani
D45N complete loss of enzyme activity, altered protein structure Leishmania donovani
E149A complete loss of enzyme activity, altered protein structure Leishmania donovani
H102N complete loss of enzyme activity, altered protein structure Leishmania donovani
H11N 10.7% loss of enzyme activity, altered protein structure Leishmania donovani
H138N 82% loss of enzyme activity, altered protein structure Leishmania donovani
P47A 30.7% loss of enzyme activity, altered protein structure Leishmania donovani
Y46F similar kinetic behaviour as wild type, but altered protein structure Leishmania donovani

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.51
-
D-ribose 5-phosphate wild-type, pH 7.5, 22°C Leishmania donovani
2.59
-
D-ribose 5-phosphate mutant Y46F, pH 7.5, 22°C Leishmania donovani
3.4
-
D-ribose 5-phosphate mutant H11N, pH 7.5, 22°C Leishmania donovani
4.3
-
D-ribose 5-phosphate mutant P47A, pH 7.5, 22°C Leishmania donovani
8.14
-
D-ribose 5-phosphate mutant H138N, pH 7.5, 22°C Leishmania donovani

Organism

Organism UniProt Comment Textmining
Leishmania donovani G9JM00
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-allose
-
Leishmania donovani D-psicose
-
?
D-Arabinose
-
Leishmania donovani ?
-
?
D-ribose
-
Leishmania donovani D-ribulose
-
?
D-ribose 5-phosphate
-
Leishmania donovani D-ribulose 5-phosphate
-
?
D-talose
-
Leishmania donovani D-tagatose
-
?
additional information enzyme exhibits activity with D-form of various aldose substrates in the order of D-ribose > D-talose > D-allose > D-arabinose Leishmania donovani ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11
-
D-ribose 5-phosphate mutant H138N, pH 7.5, 22°C Leishmania donovani
18
-
D-ribose 5-phosphate mutant P47A, pH 7.5, 22°C Leishmania donovani
25
-
D-ribose 5-phosphate mutant Y46F, pH 7.5, 22°C Leishmania donovani
29
-
D-ribose 5-phosphate wild-type, pH 7.5, 22°C Leishmania donovani
32
-
D-ribose 5-phosphate mutant H11N, pH 7.5, 22°C Leishmania donovani

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.35
-
D-ribose 5-phosphate mutant H138N, pH 7.5, 22°C Leishmania donovani
4.24
-
D-ribose 5-phosphate mutant P47A, pH 7.5, 22°C Leishmania donovani
9.51
-
D-ribose 5-phosphate mutant H11N, pH 7.5, 22°C Leishmania donovani
9.82
-
D-ribose 5-phosphate mutant Y46F, pH 7.5, 22°C Leishmania donovani
11.175
-
D-ribose 5-phosphate wild-type, pH 7.5, 22°C Leishmania donovani