Cloned (Comment) | Organism |
---|---|
- |
Leishmania donovani |
Crystallization (Comment) | Organism |
---|---|
homology modeling of wild type and mutants | Leishmania donovani |
Protein Variants | Comment | Organism |
---|---|---|
C69S | complete loss of enzyme activity, altered protein structure | Leishmania donovani |
D45N | complete loss of enzyme activity, altered protein structure | Leishmania donovani |
E149A | complete loss of enzyme activity, altered protein structure | Leishmania donovani |
H102N | complete loss of enzyme activity, altered protein structure | Leishmania donovani |
H11N | 10.7% loss of enzyme activity, altered protein structure | Leishmania donovani |
H138N | 82% loss of enzyme activity, altered protein structure | Leishmania donovani |
P47A | 30.7% loss of enzyme activity, altered protein structure | Leishmania donovani |
Y46F | similar kinetic behaviour as wild type, but altered protein structure | Leishmania donovani |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.51 | - |
D-ribose 5-phosphate | wild-type, pH 7.5, 22°C | Leishmania donovani | |
2.59 | - |
D-ribose 5-phosphate | mutant Y46F, pH 7.5, 22°C | Leishmania donovani | |
3.4 | - |
D-ribose 5-phosphate | mutant H11N, pH 7.5, 22°C | Leishmania donovani | |
4.3 | - |
D-ribose 5-phosphate | mutant P47A, pH 7.5, 22°C | Leishmania donovani | |
8.14 | - |
D-ribose 5-phosphate | mutant H138N, pH 7.5, 22°C | Leishmania donovani |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania donovani | G9JM00 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-allose | - |
Leishmania donovani | D-psicose | - |
? | |
D-Arabinose | - |
Leishmania donovani | ? | - |
? | |
D-ribose | - |
Leishmania donovani | D-ribulose | - |
? | |
D-ribose 5-phosphate | - |
Leishmania donovani | D-ribulose 5-phosphate | - |
? | |
D-talose | - |
Leishmania donovani | D-tagatose | - |
? | |
additional information | enzyme exhibits activity with D-form of various aldose substrates in the order of D-ribose > D-talose > D-allose > D-arabinose | Leishmania donovani | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
D-ribose 5-phosphate | mutant H138N, pH 7.5, 22°C | Leishmania donovani | |
18 | - |
D-ribose 5-phosphate | mutant P47A, pH 7.5, 22°C | Leishmania donovani | |
25 | - |
D-ribose 5-phosphate | mutant Y46F, pH 7.5, 22°C | Leishmania donovani | |
29 | - |
D-ribose 5-phosphate | wild-type, pH 7.5, 22°C | Leishmania donovani | |
32 | - |
D-ribose 5-phosphate | mutant H11N, pH 7.5, 22°C | Leishmania donovani |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.35 | - |
D-ribose 5-phosphate | mutant H138N, pH 7.5, 22°C | Leishmania donovani | |
4.24 | - |
D-ribose 5-phosphate | mutant P47A, pH 7.5, 22°C | Leishmania donovani | |
9.51 | - |
D-ribose 5-phosphate | mutant H11N, pH 7.5, 22°C | Leishmania donovani | |
9.82 | - |
D-ribose 5-phosphate | mutant Y46F, pH 7.5, 22°C | Leishmania donovani | |
11.175 | - |
D-ribose 5-phosphate | wild-type, pH 7.5, 22°C | Leishmania donovani |