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Literature summary for 5.3.1.6 extracted from

  • Sun, F.; Zhang, X.Z.; Myung, S.; Zhang, Y.H.
    Thermophilic Thermotoga maritima ribose-5-phosphate isomerase RpiB: optimized heat treatment purification and basic characterization (2012), Protein Expr. Purif., 82, 302-307.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Thermotoga maritima

General Stability

General Stability Organism
maximum activities in sodium dihydrogen phosphate-ciitric acid buffer at pH 6.5-8.0 and in 50 mM Tris-HCl buffer pH 7.0-9.0. This enzyme retains about 65% activity from pH 8.8-11.0 in glycine-NaOH buffer Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1 2.1 D-ribose 5-phosphate at 80°C and pH 7.0 Thermotoga maritima
7.6
-
D-ribose 5-phosphate at 60°C and pH 7.0 Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15867
-
x * 15867, calculated from amino acid sequence Thermotoga maritima
16000
-
x * 16000, SDS-PAGE Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-ribose 5-phosphate Thermotoga maritima
-
D-ribulose 5-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
-
-

Purification (Commentary)

Purification (Comment) Organism
heat precipitation Thermotoga maritima

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
187
-
cell lysate, at 50°C and pH 7.0 Thermotoga maritima
290
-
after purification, at 50°C and pH 7.0 Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribose 5-phosphate
-
Thermotoga maritima D-ribulose 5-phosphate
-
r

Subunits

Subunits Comment Organism
? x * 16000, SDS-PAGE Thermotoga maritima
? x * 15867, calculated from amino acid sequence Thermotoga maritima

Synonyms

Synonyms Comment Organism
ribose-5-phosphate isomerase type B
-
Thermotoga maritima
RpiB
-
Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 99 enzyme activity increases significantly when the temperature increases until 80°C. The optimum temperature is 80°C. The activity at 30°C is 25% of that at 80°C. The enzyme activity decreases after 80°C. It is still highly active when the temperature reaches 99°C, 90% activity of that of 80°C. This enzyme shows relatively high activity (above 50% of its maximum activity) over a large temperature range of 40-99°C Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 80 the purified enzyme at 0.1 mg/ml is very stable at 60 and 70°C but is deactivated at 80°C. The enzyme shows half lifetimes of 70.8, 53.1, 6.4, and 1.2 h at 60, 70, 80, and 90°C, respectively Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
540
-
D-ribose 5-phosphate at 60°C and pH 7.0 Thermotoga maritima
1192
-
D-ribose 5-phosphate at 80°C and pH 7.0 Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 8
-
Thermotoga maritima