Literature summary for 5.3.1.6 extracted from

Stern, A.L.; Naworyta, A.; Cazzulo, J.J.; Mowbray, S.L.
Structures of type B ribose 5-phosphate isomerase from Trypanosoma cruzi shed light on the determinants of sugar specificity in the structural family (2011), FEBS J., 278, 793-808.

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli ER2566 cells	Trypanosoma cruzi

Crystallization (Commentary)

Crystallization (Comment)	Organism
RpiB (wild type or C69A mutant enzyme), sitting drop vapor diffusion method, using 0.5% (v/v) Jeffamine ED-2001, 0.1 M HEPES, 1.1 M Na-malonate (pH 7.0) for the wild type enzyme in complex with phosphate, or 0.8 M Na?K phosphate (pH 8.2) for the mutant enzyme C69A in complex with phosphate, or 20% (w/v) poly(ethyleneglycol) 550 monomethyl ether, 0.1 M NaCl, 0.1 M bicine (pH 9.0) for the wild type enzyme in complex with D-ribose 5-phosphate, or 20% (w/v) poly(ethylene glycol) 6000, 0.2 M ammonium chloride, 0.1 M Tris-HCl (pH 8.0) for wild type enzyme in complex with 4-phospho-D-erythronohydroxamic acid, or 20% (w/v) poly(ethylene glycol) 3350, 0.2 M sodium acetate, 0.1 M bis-Tris propane (pH 8.5) for mutant enzyme C69A in complex with allose 6-phosphate	Trypanosoma cruzi

Crystallization (Comment)

Organism

RpiB (wild type or C69A mutant enzyme), sitting drop vapor diffusion method, using 0.5% (v/v) Jeffamine ED-2001, 0.1 M HEPES, 1.1 M Na-malonate (pH 7.0) for the wild type enzyme in complex with phosphate, or 0.8 M Na?K phosphate (pH 8.2) for the mutant enzyme C69A in complex with phosphate, or 20% (w/v) poly(ethyleneglycol) 550 monomethyl ether, 0.1 M NaCl, 0.1 M bicine (pH 9.0) for the wild type enzyme in complex with D-ribose 5-phosphate, or 20% (w/v) poly(ethylene glycol) 6000, 0.2 M ammonium chloride, 0.1 M Tris-HCl (pH 8.0) for wild type enzyme in complex with 4-phospho-D-erythronohydroxamic acid, or 20% (w/v) poly(ethylene glycol) 3350, 0.2 M sodium acetate, 0.1 M bis-Tris propane (pH 8.5) for mutant enzyme C69A in complex with allose 6-phosphate

Trypanosoma cruzi

Protein Variants

Protein Variants	Comment	Organism
C69A	inactive	Trypanosoma cruzi

Inhibitors

Inhibitors	Comment	Organism	Structure
D-Allose 6-phosphate	weak competitive inhibitor	Trypanosoma cruzi

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
5	-	D-ribulose 5-phosphate	in 50 mM Tris-HCl (pH 7.6), 150 mM NaCl and 5 mM MESNA, at 30°C	Trypanosoma cruzi

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma cruzi	Q4CQE2	-	-
Trypanosoma cruzi CL Brener	Q4CQE2	-	-

Purification (Commentary)

Purification (Comment)	Organism
Superdex 75 gel filtration	Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
D-ribulose 5-phosphate	-	Trypanosoma cruzi	D-ribose 5-phosphate	-	?
D-ribulose 5-phosphate	-	Trypanosoma cruzi CL Brener	D-ribose 5-phosphate	-	?
additional information	the enzyme cannot isomerizes D-allose 6-phosphate	Trypanosoma cruzi	?	-	?
additional information	the enzyme cannot isomerizes D-allose 6-phosphate	Trypanosoma cruzi CL Brener	?	-	?

Subunits

Subunits	Comment	Organism
tetramer	x-ray crystallography	Trypanosoma cruzi

Synonyms

Synonyms	Comment	Organism
ribose-5-phosphate isomerase	-	Trypanosoma cruzi
RpiB	-	Trypanosoma cruzi
type B ribose 5-phosphate isomerase	-	Trypanosoma cruzi

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2	8	D-ribulose 5-phosphate	in 50 mM Tris-HCl (pH 7.6), 150 mM NaCl and 5 mM MESNA, at 30°C	Trypanosoma cruzi

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
15	-	D-Allose 6-phosphate	in 50 mM Tris-HCl (pH 7.6), 150 mM NaCl and 5 mM MESNA, at 30°C	Trypanosoma cruzi