Cloned (Comment) | Organism |
---|---|
gene rpiA, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) | Vibrio vulnificus |
Crystallization (Comment) | Organism |
---|---|
open form RpiA in complex with substrate ribose 5-phosphate, the closed form complexed with arabinose-5-phosphate, and the apo-RpiA, hanging drop vapor diffusion method, 0.002 ml of 30 mg/ml protein in 20 mM HEPES, pH 7.5, and 150 mM KCl are mixed with 0.002 ml of a reservoir solution containing 50 mM succinate, pH 4.1, 180 mM ammonium sulfate, and 8% PEG 4000, 3 days, 20°C, for complexed enzyme addition of 20 mM ligand, X-ray diffraction structure determination and analysis at 1.49-2.07 A resolution, molecular replacement | Vibrio vulnificus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arabinose 5-phosphate | RpiA binds arabinose 5-phosphate located at the opening of the active site, the sugar ring of the inhibitor interacts with the Asp4, Lys7, Ser30, Asp118, and Lys121 residues, the phosphate group of arabinose 5-phosphate interacts with two water molecules, W51 and W82, binding mode of inhibitor, overview | Vibrio vulnificus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
recombinant detagged RpiA, dynamic light scattering and gel filtration | Vibrio vulnificus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribose 5-phosphate | Vibrio vulnificus | - |
D-ribulose 5-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio vulnificus | Q7MHL9 | gene rpiA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged RpiA from Escherichia coli strain BL21(DE3) by nickel affinity chromatography. The His-tag is cleaved off and removed, folowed by anion exchange chromatography and gel filtration | Vibrio vulnificus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribose 5-phosphate | - |
Vibrio vulnificus | D-ribulose 5-phosphate | - |
r | |
D-ribose 5-phosphate | binding mode of substrate, overview | Vibrio vulnificus | D-ribulose 5-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 20000-25000, RpiA | Vibrio vulnificus |
Synonyms | Comment | Organism |
---|---|---|
ribose 5-phosphate isomerase A | - |
Vibrio vulnificus |
RpiA | - |
Vibrio vulnificus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Vibrio vulnificus | isoelectric focusing | - |
4.77 |