Literature summary for 5.3.1.6 extracted from

Park, C.S.; Yeom, S.J.; Kim, H.J.; Lee, S.H.; Lee, J.K.; Kim, S.W.; Oh, D.K.
Characterization of ribose-5-phosphate isomerase of Clostridium thermocellum producing D-allose from D-psicose (2007), Biotechnol. Lett., 29, 1387-1391.

Search for more literature for 5.3.1.6

Application

Application	Comment	Organism
synthesis	production of D-allose from D-psicose by enzyme. At pH 7.5 and 50°C, synthesis of 165 g D-allose per l within 6 h	Acetivibrio thermocellus

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Acetivibrio thermocellus

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	EDTA is not inhibitory	Acetivibrio thermocellus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	no activation by mono- or divalent cations	Acetivibrio thermocellus

Organism

Organism	UniProt	Comment	Textmining
Acetivibrio thermocellus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-allose	substrates D-allose and D-psicose are preferred over ribose 5-phosphate	Acetivibrio thermocellus	D-psicose	-	r
D-psicose	substrates D-allose and D-psicose are preferred over ribose 5-phosphate	Acetivibrio thermocellus	D-allose	-	r
D-ribose 5-diphosphate	substrates D-allose and D-psicose are preferred over ribose 5-phosphate	Acetivibrio thermocellus	D-ribulose 5-diphosphate	-	r
additional information	no substrate: D-xylose, L-rhamnose, D-altrose, D-galactose	Acetivibrio thermocellus	?	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
65	-	optimum for synthesis of D-allose	Acetivibrio thermocellus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	half-life 96 h	Acetivibrio thermocellus
65	-	half-life 4.7 h	Acetivibrio thermocellus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	optimum for synthesis of D-allose	Acetivibrio thermocellus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)