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Literature summary for 5.3.1.5 extracted from
- L-arabinose isomerase and D-xylose isomerase from Lactobacillus reuteri: Characterization, coexpression in the food grade host Lactobacillus plantarum, and application in the conversion of D-galactose and D-glucose (2014), J. Agric. Food Chem., 62, 1617-1624.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 Star (DE3) cells and in Lactobacillus plantarum | Limosilactobacillus reuteri |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 177.4 | - |
D-xylose | at pH 5.0 and 65°C | Limosilactobacillus reuteri |  |
| 1099 | - |
D-glucose | at pH 5.0 and 65°C | Limosilactobacillus reuteri | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | with D-xylose as substrate, the activity is increased by the addition of 1 mM Co2+ (1.9fold). A mixture of 0.5 mM Co2+ and 0.5 mM Mn2+ increases the enzyme activity 3.2fold. Glucose isomerase activity is maximally enhanced by the addition of 5 mM Co2+ | Limosilactobacillus reuteri | |
| Mg2+ | glucose isomerase activity is maximally enhanced by the addition of 5 mM Mg2+ | Limosilactobacillus reuteri | |
| Mn2+ | with D-xylose as substrate, the activity is increased by the addition of 1 mM Mn2+ (2.9fold). A mixture of 0.5 mM Co2+ and 0.5 mM Mn2+ increases the enzyme activity 3.2fold. Glucose isomerase activity is maximally enhanced by the addition of 5 mM Mn2+ | Limosilactobacillus reuteri | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51040 | - |
4 * 51040, calculated from amino acid sequence | Limosilactobacillus reuteri |
| 190000 | - |
gel filtration | Limosilactobacillus reuteri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Xylose | Limosilactobacillus reuteri | - |
D-Xylulose | - |
r | |
| D-Xylose | Limosilactobacillus reuteri DSM Z 17509 | - |
D-Xylulose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Limosilactobacillus reuteri | - |
- |
- |
| Limosilactobacillus reuteri DSM Z 17509 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HisPrep FF 16/10 column chromatography | Limosilactobacillus reuteri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.57 | - |
using D-glucose as substrate, at pH 5.0 and 65°C | Limosilactobacillus reuteri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Glucose | - |
Limosilactobacillus reuteri | D-Fructose | maximum conversion rate of 48% at 60°C | r | |
| D-Glucose | - |
Limosilactobacillus reuteri DSM Z 17509 | D-Fructose | maximum conversion rate of 48% at 60°C | r | |
| D-Xylose | - |
Limosilactobacillus reuteri | D-Xylulose | - |
r | |
| D-Xylose | - |
Limosilactobacillus reuteri DSM Z 17509 | D-Xylulose | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 51040, calculated from amino acid sequence | Limosilactobacillus reuteri |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-XI | - |
Limosilactobacillus reuteri |
| D-xylose isomerase | - |
Limosilactobacillus reuteri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Limosilactobacillus reuteri |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 80 | about 30% activity at 50 and 80°C and about 85% activity at 60 and 70°C | Limosilactobacillus reuteri |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 15.5 | - |
D-glucose | at pH 5.0 and 65°C | Limosilactobacillus reuteri | |
| 146.6 | - |
D-xylose | at pH 5.0 and 65°C | Limosilactobacillus reuteri | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Limosilactobacillus reuteri |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.014 | - |
D-glucose | at pH 5.0 and 65°C | Limosilactobacillus reuteri | |
| 0.83 | - |
D-xylose | at pH 5.0 and 65°C | Limosilactobacillus reuteri | |
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