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Literature summary for 5.3.1.5 extracted from

  • Lee, S.M.; Jellison, T.; Alper, H.S.
    Directed evolution of xylose isomerase for improved xylose catabolism and fermentation in the yeast Saccharomyces cerevisiae (2012), Appl. Environ. Microbiol., 78, 5708-5716.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain BY4741-S1 Piromyces sp.

Protein Variants

Protein Variants Comment Organism
E129D/V433I the mutation shows an increase in enzymatic activity Piromyces sp.
E129D/V433I/E15D/E114G the mutation shows an increase in enzymatic activity Piromyces sp.
E129D/V433I/E15D/E114G/T142S/A177T the mutant exhibits a 77% increase in enzymatic activity. A yeast strain expressing this mutant enzyme improves its aerobic growth rate by 61fold and both ethanol production and xylose consumption rates by nearly 8fold Piromyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
50.18
-
 D-xylose mutant enzyme E129D/V433I/E15D/E114G, in 100 mM Tris-HCl buffer at pH 7.5 and 30°C Piromyces sp.
86.97
-
 D-xylose wild type enzyme, in 100 mM Tris-HCl buffer at pH 7.5 and 30°C Piromyces sp.
89.98
-
 D-xylose mutant enzyme E129D/V433I, in 100 mM Tris-HCl buffer at pH 7.5 and 30°C Piromyces sp.
168.4
-
 D-xylose mutant enzyme E129D/V433I/E15D/E114G/T142S/A177T, in 100 mM Tris-HCl buffer at pH 7.5 and 30°C Piromyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-Xylose Piromyces sp.
-
 D-Xylulose
-
 r

Organism

Organism UniProt Comment Textmining
Piromyces sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Xylose
-
 Piromyces sp. D-Xylulose
-
 r

Synonyms

Synonyms Comment Organism
XYLA
-
 Piromyces sp.