Literature summary for 5.3.1.5 extracted from

Cho, J.W.; Han, B.G.; Park, S.Y.; Kim, S.J.; Kim, M.D.; Lee, B.I.
Overexpression, crystallization and preliminary X-ray crystallographic analysis of a putative xylose isomerase from Bacteroides thetaiotaomicron (2013), Acta Crystallogr. Sect. F, 69, 1127-1130.

Search for more literature for 5.3.1.5

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta2 (DE3)pLysS cells	Bacteroides thetaiotaomicron

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 100 mM sodium chloride, 100 mM bicine pH 9.0, 20% (v/v) PEG MME 550	Bacteroides thetaiotaomicron

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50000	-	x * 50000, SDS-PAGE	Bacteroides thetaiotaomicron

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-Glucose	Bacteroides thetaiotaomicron	-	D-Fructose	-	r
D-Glucose	Bacteroides thetaiotaomicron DSM 2079	-	D-Fructose	-	r
D-Xylose	Bacteroides thetaiotaomicron	-	D-Xylulose	-	r
D-Xylose	Bacteroides thetaiotaomicron DSM 2079	-	D-Xylulose	-	r

Organism

Organism	UniProt	Comment	Textmining
Bacteroides thetaiotaomicron	Q8A9M2	-	-
Bacteroides thetaiotaomicron DSM 2079	Q8A9M2	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, Ni-NTA column chromatography, and Superdex 200 gel filtration	Bacteroides thetaiotaomicron

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-Glucose	-	Bacteroides thetaiotaomicron	D-Fructose	-	r
D-Glucose	-	Bacteroides thetaiotaomicron DSM 2079	D-Fructose	-	r
D-Xylose	-	Bacteroides thetaiotaomicron	D-Xylulose	-	r
D-Xylose	-	Bacteroides thetaiotaomicron DSM 2079	D-Xylulose	-	r

Subunits

Subunits	Comment	Organism
?	x * 50000, SDS-PAGE	Bacteroides thetaiotaomicron

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Release 2023.1 (January 2023)