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Literature summary for 5.3.1.5 extracted from

  • Kovalevsky, A.Y.; Katz, A.K.; Carrell, H.L.; Hanson, L.; Mustyakimov, M.; Fisher, S.Z.; Coates, L.; Schoenborn, B.P.; Bunick, G.J.; Glusker, J.P.; Langan, P.
    Hydrogen location in stages of an enzyme-catalyzed reaction: time-of-flight neutron structure of D-xylose isomerase with bound D-xylulose (2008), Biochemistry, 47, 7595-7597.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
analysis of the location of hydrogen atoms by time-of-flight neutron Laue technique. The neutron structure of crystalline XI with bound product, D-xylulose, shows, that O5 of D-xylulose is not protonated but is hydrogen-bonded to doubly protonated His54. Also, Lys289, which is neutral in native XI, is protonated, while the catalytic water in native XI has become activated to a hydroxyl anion which is in the proximity of C1 and C2, the molecular site of isomerization of xylose Streptomyces rubiginosus

Organism

Organism UniProt Comment Textmining
Streptomyces rubiginosus P24300
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