Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Actinoplanes missouriensis |
Crystallization (Comment) | Organism |
---|---|
- |
Actinoplanes missouriensis |
- |
Streptomyces rubiginosus |
Protein Variants | Comment | Organism |
---|---|---|
F26W | catalytic efficiency with L-arabinose is increased 2fold | Actinoplanes missouriensis |
Q256D | catalytic efficiency with L-arabinose is increased 3fold, reaction rate with L-ribose is increased 6fold | Actinoplanes missouriensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
900 | - |
L-arabinose | mutant Q256D | Actinoplanes missouriensis | |
1400 | - |
L-arabinose | mutant F26W | Actinoplanes missouriensis | |
1500 | - |
L-arabinose | wild-type | Actinoplanes missouriensis | |
1600 | - |
D-glucose | mutant F26W | Actinoplanes missouriensis | |
1900 | - |
D-glucose | wild-type | Actinoplanes missouriensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Xylose | Actinoplanes missouriensis | - |
D-Xylulose | - |
? | |
D-Xylose | Streptomyces rubiginosus | - |
D-Xylulose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Actinoplanes missouriensis | - |
- |
- |
Streptomyces rubiginosus | P24300 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Actinoplanes missouriensis |
- |
Streptomyces rubiginosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glucose | - |
Actinoplanes missouriensis | D-Fructose | - |
? | |
D-Glucose | - |
Streptomyces rubiginosus | D-Fructose | - |
? | |
D-Xylose | - |
Actinoplanes missouriensis | D-Xylulose | - |
? | |
D-Xylose | - |
Streptomyces rubiginosus | D-Xylulose | - |
? | |
L-arabinose | - |
Actinoplanes missouriensis | L-ribulose | - |
r | |
L-arabinose | low reaction efficiency | Streptomyces rubiginosus | L-ribulose | - |
r | |
L-ribose | no increased reaction rate observed for mutant F26W | Actinoplanes missouriensis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha4, crystal structure analysis | Streptomyces rubiginosus |
Synonyms | Comment | Organism |
---|---|---|
XI | - |
Actinoplanes missouriensis |
XI | - |
Streptomyces rubiginosus |
xylose isomerase | - |
Actinoplanes missouriensis |
xylose isomerase | - |
Streptomyces rubiginosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
75-80°C for mutant F26W | Actinoplanes missouriensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
mutant Q256D remains fully active for 60 min at 65°C, whereas the activity is rapidly lost at 80°C | Actinoplanes missouriensis |
75 | - |
wild-type enzyme remains fully active for 60 min at 75°C | Actinoplanes missouriensis |
80 | - |
mutant F26W remains fully active for 60 min at 80°C | Actinoplanes missouriensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
L-arabinose | wild-type | Actinoplanes missouriensis | |
0.155 | - |
L-arabinose | mutant F26W | Actinoplanes missouriensis | |
0.158 | - |
L-arabinose | mutant Q256D | Actinoplanes missouriensis | |
20.5 | - |
D-glucose | mutant F26W | Actinoplanes missouriensis | |
25.3 | - |
D-glucose | wild-type | Actinoplanes missouriensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Actinoplanes missouriensis |