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Literature summary for 5.3.1.5 extracted from

  • Karimaki, J.; Parkkinen, T.; Santa, H.; Pastinen, O.; Leisola, M.; Rouvinen, J.; Turunen, O.
    Engineering the substrate specificity of xylose isomerase (2004), Protein Eng. Des. Sel., 17, 861-869.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Actinoplanes missouriensis

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Actinoplanes missouriensis
-
Streptomyces rubiginosus

Protein Variants

Protein Variants Comment Organism
F26W catalytic efficiency with L-arabinose is increased 2fold Actinoplanes missouriensis
Q256D catalytic efficiency with L-arabinose is increased 3fold, reaction rate with L-ribose is increased 6fold Actinoplanes missouriensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
900
-
L-arabinose mutant Q256D Actinoplanes missouriensis
1400
-
L-arabinose mutant F26W Actinoplanes missouriensis
1500
-
L-arabinose wild-type Actinoplanes missouriensis
1600
-
D-glucose mutant F26W Actinoplanes missouriensis
1900
-
D-glucose wild-type Actinoplanes missouriensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-Xylose Actinoplanes missouriensis
-
D-Xylulose
-
?
D-Xylose Streptomyces rubiginosus
-
D-Xylulose
-
?

Organism

Organism UniProt Comment Textmining
Actinoplanes missouriensis
-
-
-
Streptomyces rubiginosus P24300
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Actinoplanes missouriensis
-
Streptomyces rubiginosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Glucose
-
Actinoplanes missouriensis D-Fructose
-
?
D-Glucose
-
Streptomyces rubiginosus D-Fructose
-
?
D-Xylose
-
Actinoplanes missouriensis D-Xylulose
-
?
D-Xylose
-
Streptomyces rubiginosus D-Xylulose
-
?
L-arabinose
-
Actinoplanes missouriensis L-ribulose
-
r
L-arabinose low reaction efficiency Streptomyces rubiginosus L-ribulose
-
r
L-ribose no increased reaction rate observed for mutant F26W Actinoplanes missouriensis ?
-
?

Subunits

Subunits Comment Organism
tetramer alpha4, crystal structure analysis Streptomyces rubiginosus

Synonyms

Synonyms Comment Organism
XI
-
Actinoplanes missouriensis
XI
-
Streptomyces rubiginosus
xylose isomerase
-
Actinoplanes missouriensis
xylose isomerase
-
Streptomyces rubiginosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
75-80°C for mutant F26W Actinoplanes missouriensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
mutant Q256D remains fully active for 60 min at 65°C, whereas the activity is rapidly lost at 80°C Actinoplanes missouriensis
75
-
wild-type enzyme remains fully active for 60 min at 75°C Actinoplanes missouriensis
80
-
mutant F26W remains fully active for 60 min at 80°C Actinoplanes missouriensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
L-arabinose wild-type Actinoplanes missouriensis
0.155
-
L-arabinose mutant F26W Actinoplanes missouriensis
0.158
-
L-arabinose mutant Q256D Actinoplanes missouriensis
20.5
-
D-glucose mutant F26W Actinoplanes missouriensis
25.3
-
D-glucose wild-type Actinoplanes missouriensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
Actinoplanes missouriensis