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Literature summary for 5.3.1.5 extracted from

  • Park, J.H.; Batt, C.A.
    Restoration of a defective Lactococcus lactis xylose isomerase (2004), Appl. Environ. Microbiol., 70, 4318-4325.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Lactococcus lactis

Protein Variants

Protein Variants Comment Organism
E407K 2% of strain IO-1 wild-type activity Lactococcus lactis
K407E 92% of wild-type activity Lactococcus lactis
R202M 9% of strain IO-1 wild-type activity Lactococcus lactis
R202M/V275A 26% of strain IO-1 wild-type activity Lactococcus lactis
R202M/Y218D 9% of strain IO-1 wild-type activity Lactococcus lactis
R202M/Y218D/V275A 62% of strain IO-1 wild-type activity and soluble Lactococcus lactis
S247A 97% of wild-type activity Lactococcus lactis
S247A/K407E 79% of wild-type activity Lactococcus lactis
S247A/S388T 27% of wild-type activity Lactococcus lactis
S388T insoluble and 8% of wild-type activity Lactococcus lactis
S388T/K407E soluble and 50% of wild-type activity Lactococcus lactis
T388S 11% of strain IO-1 wild-type activity Lactococcus lactis
Y218D 14% of strain IO-1 wild-type activity Lactococcus lactis
Y218D/V275A 24% of strain IO-1 wild-type activity Lactococcus lactis
Y275A 24% of strain IO-1 wild-type activity Lactococcus lactis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.25
-
D-xylose wild-type Lactococcus lactis
3.46
-
D-xylose mutant R202M/Y218D/V275A Lactococcus lactis
4.4
-
D-xylose mutant S388T/K407E Lactococcus lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
SDS-PAGE Lactococcus lactis
49700
-
calculated from amino acid sequence Lactococcus lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-Xylose Lactococcus lactis
-
D-Xylulose
-
?
D-Xylose Lactococcus lactis IO-1 (Xyl+)
-
D-Xylulose
-
?
D-Xylose Lactococcus lactis 210 (Xyl-)
-
D-Xylulose
-
?

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
-
-
Lactococcus lactis 210 (Xyl-)
-
-
-
Lactococcus lactis IO-1 (Xyl+)
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purified enzyme is insoluble and inactive Lactococcus lactis
purified enzyme is soluble Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Xylose
-
Lactococcus lactis D-Xylulose
-
?
D-Xylose
-
Lactococcus lactis IO-1 (Xyl+) D-Xylulose
-
?
D-Xylose
-
Lactococcus lactis 210 (Xyl-) D-Xylulose
-
?

Synonyms

Synonyms Comment Organism
XI
-
Lactococcus lactis
xylose isomerase
-
Lactococcus lactis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Lactococcus lactis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
74
-
activity decreases dramatically above 74°C Lactococcus lactis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
124
-
D-xylose mutant S388T/K407E Lactococcus lactis
142
-
D-xylose mutant R202M/Y218D/V275A Lactococcus lactis
184
-
D-xylose wild-type Lactococcus lactis