Cloned (Comment) | Organism |
---|---|
wild-type and mutant enzymes, expression in Escherichia coli | Arthrobacter sp. |
Crystallization (Comment) | Organism |
---|---|
wild-type and mutant enzymes | Arthrobacter sp. |
Protein Variants | Comment | Organism |
---|---|---|
Y253C | a Tyr253 mutant in which a disulfide bridge is introduced at the A-B subunit interface shows reduced thermostability, that is identical in both oxidized and reduced forms and also reduced stability in urea. X-ray-crystallographic analysis of the Mn2+-xylitol form of oxidized Y253C shows a changed conformation of Glu185 and also alternative conformations for Asp254, which is a ligand to the site 2 metal ion. With fructose, Mg2+-Y253C has a similar Km to that of the wild-type, and its maximal velocity is also similar below pH 6.4, but declines thereafter. In presence of Co2+, Y253C has lower activity than wild-type at all pH values, but its activity also declines at alkaline pH | Arthrobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Arthrobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Xylose | - |
Arthrobacter sp. | D-Xylulose | - |
? |