Any feedback?
Literature summary for 5.3.1.5 extracted from
- Protein engineering of xylose (glucose) isomerase from Actinoplanes missouriensis. 3. Changing metal specificity and the pH profile by site-directed mutagenesis (1992), Biochemistry, 31, 5467-5471.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E186D | mutant enzymes E186D and E186Q are active, and their metal specificity is different from that of the wild type. The E186 enzyme is most active with Mn2+ and has a drastically shifted pH optimum | Actinoplanes missouriensis |
| E186Q | mutant enzymes E186D and E186Q are active, and their metal specificity is different from that of the wild type. The E186 enzyme is most active with Mn2+ and has a drastically shifted pH optimum | Actinoplanes missouriensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km-values for wild-type enzyme and mutants E186D and E186Q, activated by Mg2+, Mn2+ or Co2+ | Actinoplanes missouriensis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | Mg2+, Mn2+ or Co2+ required for maximal activity | Actinoplanes missouriensis |  |
| Mg2+ | - |
Actinoplanes missouriensis | |
| Mn2+ | - |
Actinoplanes missouriensis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Actinoplanes missouriensis | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Xylose | - |
Actinoplanes missouriensis | D-Xylulose | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | turnover numbers for wild-type enzyme and mutant enzymes E186D and E186Q, activated by Mg2+, Mn2+ or Co2+ | Actinoplanes missouriensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.25 | - |
mutant E186Q, xylose isomerization, activated by Mn2+ or Mg2+ | Actinoplanes missouriensis |
| 7.3 | - |
wild-type enzyme, xylose isomerization, activated by Mg2+ | Actinoplanes missouriensis |
| 7.9 | - |
wild-type enzyme, xylose isomerization, activated by Mg2+ | Actinoplanes missouriensis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
