Cloned (Comment) | Organism |
---|---|
wild-type and mutant enzymes, overexpression in Escherichia coli | Actinoplanes missouriensis |
Crystallization (Comment) | Organism |
---|---|
structure of a number of binary and ternary complexes involving wild-type and mutant enzymes, the divalent cations Mg2+, Co2+, or Mn2+ and either the substrate xylose or substrate analogs | Actinoplanes missouriensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.8 | - |
D-xylose | wild-type enzyme | Actinoplanes missouriensis | |
16 | - |
D-xylose | D-xylose, mutant H220Q | Actinoplanes missouriensis | |
48 | - |
D-xylose | mutant D257E | Actinoplanes missouriensis | |
49 | - |
D-xylose | mutant D257N | Actinoplanes missouriensis | |
50 | - |
D-fructose | D-xylose, mutant E181Q | Actinoplanes missouriensis | |
66 | - |
D-xylose | mutant D255N | Actinoplanes missouriensis | |
73 | - |
D-xylose | mutant E181D | Actinoplanes missouriensis | |
290 | - |
D-glucose | wild-type enzyme | Actinoplanes missouriensis | |
300 | - |
D-glucose | mutant D257N | Actinoplanes missouriensis | |
400 | - |
D-glucose | D-glucose, mutant H220Q | Actinoplanes missouriensis | |
500 | - |
D-glucose | mutant H220N | Actinoplanes missouriensis | |
2400 | - |
D-glucose | mutant E181D | Actinoplanes missouriensis | |
5800 | - |
D-glucose | mutant D257E | Actinoplanes missouriensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Actinoplanes missouriensis | |
Mg2+ | Mg2+, Mn2+ or Co2+ required for maximal activity | Actinoplanes missouriensis | |
Mn2+ | Mg2+, Mn2+ or Co2+ required for maximal activity | Actinoplanes missouriensis | |
additional information | two metal sites: metal site 1 is four-coordinated and tetrahedral in the absence of substrate and is six-coordinated and octahedral in its presence, the O2 and O4 atoms of the linear inhibitors and substrate bind to the metal 1. Metal site 2 is octahedral in all cases, its position changes by 0.7 A when it binds O1 of the substrate and by more than 1 A when it also binds O2 | Actinoplanes missouriensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Actinoplanes missouriensis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glucose | - |
Actinoplanes missouriensis | D-Fructose | - |
? | |
D-Xylose | - |
Actinoplanes missouriensis | D-Xylulose | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
D-xylose | mutant H220N | Actinoplanes missouriensis | |
0.08 | - |
D-xylose | mutant E217S | Actinoplanes missouriensis | |
0.1 | - |
D-xylose | mutant E181Q | Actinoplanes missouriensis | |
0.2 | - |
D-glucose | mutant H220N | Actinoplanes missouriensis | |
0.5 | - |
D-xylose | mutant D255N | Actinoplanes missouriensis | |
0.6 | - |
D-xylose | mutant H220Q | Actinoplanes missouriensis | |
0.7 | - |
D-glucose | mutant H220Q | Actinoplanes missouriensis | |
0.9 | - |
D-xylose | mutant D257N | Actinoplanes missouriensis | |
1.5 | - |
D-xylose | mutant E181D | Actinoplanes missouriensis | |
3.4 | - |
D-xylose | mutant D257E | Actinoplanes missouriensis | |
5 | - |
D-glucose | mutant D257N | Actinoplanes missouriensis | |
9.3 | - |
D-glucose | mutant E181D | Actinoplanes missouriensis | |
17.3 | - |
D-xylose | wild-type enzyme | Actinoplanes missouriensis | |
17.7 | - |
D-glucose | mutant D257E | Actinoplanes missouriensis | |
24.9 | - |
D-glucose | wild-type enzyme | Actinoplanes missouriensis |