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Literature summary for 5.3.1.5 extracted from

  • Hess, J.M.; Tchernajenko, V.; Vieille, C.; Zeikus, J.G.; Kelly, R.M.
    Thermotoga neapolitana homotetrameric xylose isomerase is expressed as a catalytically active and thermostable dimer in Escherichia coli (1998), Appl. Environ. Microbiol., 64, 2357-2360.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermotoga neapolitana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 2 * 50000, the recombinant enzyme exists as homodimer and homotetramer, with the dimer being the more abundant form. The native enzyme exclusively exists as tetramer Thermotoga neapolitana
50000
-
 4 * 50000, the recombinant enzyme exists as homodimer and homotetramer, with the dimer being the more abundant form. The native enzyme exclusively exists as tetramer Thermotoga neapolitana
210000
-
 gel filtration Thermotoga neapolitana

Organism

Organism UniProt Comment Textmining
Thermotoga neapolitana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermotoga neapolitana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Xylose
-
 Thermotoga neapolitana D-Xylulose
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 50000, the recombinant enzyme exists as homodimer and homotetramer, with the dimer being the more abundant form. The native enzyme exclusively exists as tetramer Thermotoga neapolitana
tetramer 4 * 50000, the recombinant enzyme exists as homodimer and homotetramer, with the dimer being the more abundant form. The native enzyme exclusively exists as tetramer Thermotoga neapolitana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
-
 Thermotoga neapolitana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Thermotoga neapolitana