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Literature summary for 5.3.1.5 extracted from
- Purification and characterization of D-xylose isomerase from Bifidobacterium adolescentis (1994), Biosci. Biotechnol. Biochem., 58, 691-694.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4 | - |
D-xylose | - |
Bifidobacterium adolescentis |  |
| 298 | - |
D-glucose | - |
Bifidobacterium adolescentis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
3 * 53000, SDS-PAGE | Bifidobacterium adolescentis |
| 168000 | - |
gel filtration | Bifidobacterium adolescentis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bifidobacterium adolescentis | - |
- |
- |
| Streptomyces phaeochromogenes | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no glycoprotein | - |
Bifidobacterium adolescentis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bifidobacterium adolescentis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.17 | - |
- |
Bifidobacterium adolescentis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Glucose | - |
Bifidobacterium adolescentis | D-Fructose | - |
r | |
| D-Xylose | - |
Bifidobacterium adolescentis | D-Xylulose | - |
? | |
| D-Xylose | - |
Streptomyces phaeochromogenes | D-Xylulose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 53000, SDS-PAGE | Bifidobacterium adolescentis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Bifidobacterium adolescentis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | 30°C: about 45% of maximal activity, 70°C: about 10% of maximal activity | Bifidobacterium adolescentis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
10 min, stable up to | Bifidobacterium adolescentis |
| 60 | - |
10 min, 20% loss of activity | Bifidobacterium adolescentis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Bifidobacterium adolescentis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | pH 6: about 70% of maximal activity, pH 9: about 90% of maximal activity | Bifidobacterium adolescentis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
8°C, 18 h, about 50% loss of activity | Bifidobacterium adolescentis |
| 7 | 8 | 8°C, 18 h, stable | Bifidobacterium adolescentis |
| 9 | - |
8°C, 18 h, about 10% loss of activity | Bifidobacterium adolescentis |
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Release 2023.1 (January 2023)
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