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Literature summary for 5.3.1.5 extracted from

  • Callens, M.; Kersters-Hilderson, H.; van Opstal, O.; de Bruyne, C.K.
    Catalytic properties of D-xylose isomerase from Streptomyces violaceoruber (1986), Enzyme Microb. Technol., 8, 696-700.
No PubMed abstract available

General Stability

General Stability Organism
Co2+ is superior as protector against thermal inactivation at 80°C Streptomyces violaceoruber

Inhibitors

Inhibitors Comment Organism Structure
D-sorbitol
-
Streptomyces violaceoruber
D-xylitol
-
Streptomyces violaceoruber
Tris(hydroxymethyl)aminomethane
-
Streptomyces violaceoruber

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.9
-
D-xylose with 10 mM Mg2+ Streptomyces violaceoruber
3.3
-
D-xylose with 1 mM Co2+ Streptomyces violaceoruber

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates Streptomyces violaceoruber
Fe2+ slight activation Streptomyces violaceoruber
Mg2+ activates, maximal activity at 10 mM Streptomyces violaceoruber
Mn2+ activates Streptomyces violaceoruber

Organism

Organism UniProt Comment Textmining
Streptomyces violaceoruber
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Glucose
-
Streptomyces violaceoruber D-Fructose
-
?
D-Xylose r Streptomyces violaceoruber D-Xylulose
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
Streptomyces violaceoruber

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
Co2+ is superior as protector against thermal inactivation at 80°C Streptomyces violaceoruber
60
-
30 days, stable in presence of 1 mM Co2+ or 10 mM Mg2+ Streptomyces violaceoruber

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8
-
D-xylose with 1 mM Co2+ Streptomyces violaceoruber
4.15
-
D-xylose with 10 mM Mg2+ Streptomyces violaceoruber

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 9.5
-
Streptomyces violaceoruber