Any feedback?
Literature summary for 5.3.1.4 extracted from
- Engineering the L-arabinose isomerase from Enterococcus faecium for D-tagatose synthesis (2017), Molecules, 22, 2164 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Enterococcus faecium |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | expression of N- and C-terminal His-tagged protein in Escherichia coli. The C-His-tagged enzyme is preferentially hexameric in solution, whereas the N-His-tagged protein is mainly monomeric. The N-His-tagged variant shows a maximum bioconversion yield of 26% at 50°C for D-tagatose biosynthesis, the C-His-tagged variant is more active and stable at alkaline pH than the N-His-tagged variant | Enterococcus faecium |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 252 | - |
D-galactose | N-His-tagged recombinant protein, Michaelis-Menten kinetics, pH 5.6, 50°C | Enterococcus faecium |  |
| 426 | - |
D-galactose | C-His-tagged recombinant protein, K0.5 value, cooperative interaction model, pH 5.6, 50°C | Enterococcus faecium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28400 | - |
plus 129000 and 53000, C-His-tagged recombinant protein, gel filtration | Enterococcus faecium |
| 52000 | - |
plus 181000 and 311000, N-His-tagged recombinant protein, gel filtration | Enterococcus faecium |
| 53000 | - |
plus 129000 and 284000, C-His-tagged recombinant protein, gel filtration | Enterococcus faecium |
| 129000 | - |
plus 284000 and 53000, C-His-tagged recombinant protein, gel filtration | Enterococcus faecium |
| 181000 | - |
plus 311000 and 52000, N-His-tagged recombinant protein, gel filtration | Enterococcus faecium |
| 311000 | - |
plus 181000 and 52000, N-His-tagged recombinant protein, gel filtration | Enterococcus faecium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterococcus faecium | A0A1L2F0V9 | - |
- |
| Enterococcus faecium DBFIQ E36 | A0A1L2F0V9 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.86 | - |
substrate D-galactose, C-His-tagged recombinant protein, pH 5.6, 50°C | Enterococcus faecium |
| 2.77 | - |
substrate D-galactose, N-His-tagged recombinant protein, pH 5.6, 50°C | Enterococcus faecium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactose | - |
Enterococcus faecium | D-tagatose | - |
? | |
| D-galactose | - |
Enterococcus faecium DBFIQ E36 | D-tagatose | - |
? | |
| L-arabinose | - |
Enterococcus faecium | L-ribulose | - |
? | |
| L-arabinose | - |
Enterococcus faecium DBFIQ E36 | L-ribulose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
wild-type, presence of Mn2+ | Enterococcus faecium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.6 | - |
wild-type, presence of Mn2+ | Enterococcus faecium |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
