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Literature summary for 5.3.1.4 extracted from
- Cloning, expression, and characterization of a novel L-arabinose isomerase from the psychrotolerant bacterium Pseudoalteromonas haloplanktis (2016), Mol. Biotechnol., 58, 695-706 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pseudoalteromonas haloplanktis |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| homology modeling of structure | Pseudoalteromonas haloplanktis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 111.7 | - |
L-arabinose | pH 7.0, 40°C | Pseudoalteromonas haloplanktis |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 1 mM, 4.2fold activation | Pseudoalteromonas haloplanktis | |
| Mn2+ | 1 mM, 3.4fold activation | Pseudoalteromonas haloplanktis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudoalteromonas haloplanktis | Q3IG38 | - |
- |
| Pseudoalteromonas haloplanktis ATCC 14393 | Q3IG38 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.2 | - |
substrate L-arabinose, pH 7.0, 40°C | Pseudoalteromonas haloplanktis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arabinose | - |
Pseudoalteromonas haloplanktis | L-ribulose | - |
? | |
| L-arabinose | - |
Pseudoalteromonas haloplanktis ATCC 14393 | L-ribulose | - |
? | |
| additional information | no substrate: D-galactose | Pseudoalteromonas haloplanktis | ? | - |
? | |
| additional information | no substrate: D-galactose | Pseudoalteromonas haloplanktis ATCC 14393 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, SDS-PAGE, x * 55014, calculated from sequence | Pseudoalteromonas haloplanktis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Pseudoalteromonas haloplanktis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
94% of maximum activity | Pseudoalteromonas haloplanktis |
| 60 | - |
15% of maximum activity | Pseudoalteromonas haloplanktis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
2 h, 80% residual activity | Pseudoalteromonas haloplanktis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12.9 | - |
L-arabinose | pH 7.0, 40°C | Pseudoalteromonas haloplanktis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Pseudoalteromonas haloplanktis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9 | more than 75% of maximum activity | Pseudoalteromonas haloplanktis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
L-arabinose | pH 7.0, 40°C | Pseudoalteromonas haloplanktis | |
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