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Literature summary for 5.3.1.4 extracted from

  • Mei, W.; Wang, L.; Zang, Y.; Zheng, Z.; Ouyang, J.
    Characterization of an L-arabinose isomerase from Bacillus coagulans NL01 and its application for D-tagatose production (2016), BMC Biotechnol., 16, 55 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis under optimal conditions, recombinant Escherichia coli cells expressing AraA can convert 150 g/l and 250 g/l D-galactose to D-tagatose with conversion rates of 32% (32 h) and 27% (48 h) Weizmannia coagulans

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Weizmannia coagulans

Inhibitors

Inhibitors Comment Organism Structure
EDTA 60% loss of activity Weizmannia coagulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
269.8
-
L-arabinose pH 7.5, 60°C Weizmannia coagulans
355.1
-
D-galactose pH 7.5, 60°C Weizmannia coagulans

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 0.5 mM, 190% of the activity of EDTA-treated enzyme Weizmannia coagulans
Mn2+ 0.5 mM, 270% of the activity of EDTA-treated enzyme Weizmannia coagulans

Organism

Organism UniProt Comment Textmining
Weizmannia coagulans A0A191W0D9
-
-
Weizmannia coagulans NL01 A0A191W0D9
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1
-
substrate D-galactose, pH 7.5, 60°C Weizmannia coagulans
8
-
substrate L-arabinose, pH 7.5, 60°C Weizmannia coagulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose
-
Weizmannia coagulans D-tagatose
-
?
D-galactose
-
Weizmannia coagulans NL01 D-tagatose
-
?
L-arabinose
-
Weizmannia coagulans L-ribulose
-
?
L-arabinose
-
Weizmannia coagulans NL01 L-ribulose
-
?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE, x * 53500, calculated from sequence Weizmannia coagulans

Synonyms

Synonyms Comment Organism
AraA
-
Weizmannia coagulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Weizmannia coagulans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 70 more than 85% of maximum activity Weizmannia coagulans
80
-
above 60% of maximum activity Weizmannia coagulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Weizmannia coagulans

pH Range

pH Minimum pH Maximum Comment Organism
8 9 more than 90% of maximum activity Weizmannia coagulans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.017
-
D-galactose pH 7.5, 60°C Weizmannia coagulans
0.145
-
L-arabinose pH 7.5, 60°C Weizmannia coagulans