Literature summary for 5.3.1.4 extracted from

Choi, J.M.; Lee, Y.J.; Cao, T.P.; Shin, S.M.; Park, M.K.; Lee, H.S.; di Luccio, E.; Kim, S.B.; Lee, S.J.; Lee, S.J.; Lee, S.H.; Lee, D.W.
Structure of the thermophilic L-arabinose isomerase from Geobacillus kaustophilus reveals metal-mediated intersubunit interactions for activity and thermostability (2016), Arch. Biochem. Biophys., 596, 51-62 .

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Geobacillus kaustophilus

Crystallization (Commentary)

Crystallization (Comment)	Organism
structures of apo and holo forms. The substrate-binding site of GKAI is formed through displacement of residues at the intersubunit interface when it is bound to Mn2+	Geobacillus kaustophilus

Inhibitors

Inhibitors	Comment	Organism	Structure
Co2+	58% of the activity with Mn2+	Geobacillus kaustophilus
EDTA	1 mM, 25% of the activity in presence of 1 mM Mn2+	Geobacillus kaustophilus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
11.3	-	L-arabinose	pH 7.5, 22°C	Geobacillus kaustophilus
86.1	-	D-galactose	pH 7.5, 22°C	Geobacillus kaustophilus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	106% of the activity with Mn2+	Geobacillus kaustophilus
Mg2+	117% of the activity with Mn2+	Geobacillus kaustophilus
Mn2+	presence of Mn2+ stabilizes protein 1.9- and 9fold at 60°C and 70°C, respectively	Geobacillus kaustophilus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
336000	-	gel filtration	Geobacillus kaustophilus

Organism

Organism	UniProt	Comment	Textmining
Geobacillus kaustophilus	Q5KYP7	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-galactose	-	Geobacillus kaustophilus	D-tagatose	-	?
L-arabinose	-	Geobacillus kaustophilus	L-ribulose	-	?

Subunits

Subunits	Comment	Organism
hexamer	6 * 56000, SDS-PAGE	Geobacillus kaustophilus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	presence of Mn2+	Geobacillus kaustophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	presence of Mn2+, stable for at least 2 h	Geobacillus kaustophilus
70	-	presence of Mn2+, half-life 29.2 min	Geobacillus kaustophilus

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Release 2023.1 (January 2023)