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Literature summary for 5.3.1.4 extracted from

  • Fan, C.; Xu, W.; Zhang, T.; Zhou, L.; Jiang, B.; Mu, W.
    Engineering of Alicyclobacillus hesperidum L-arabinose isomerase for improved catalytic activity and reduced pH optimum using random and site-directed mutagenesis (2015), Appl. Biochem. Biotechnol., 177, 1480-1492 .
    View publication on PubMed
Search for more literature for 5.3.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Alicyclobacillus hesperidum

Protein Variants

Protein Variants Comment Organism
D478A mutant retains more than 80 % of the maximum relative activity of the wild-type at 75°C Alicyclobacillus hesperidum
D478K mutant displays a decreased optimum pH value Alicyclobacillus hesperidum
D478N mutant shows increased activity for D-galactose isomerization and retains more than 80 % of the maximum relative activity of the wild-type at 75°C. Mutant displays a decreased optimum pH value Alicyclobacillus hesperidum
D478Q mutant shows increased activity for D-galactose isomerization and retains more than 80 % of the maximum relative activity of the wild-type at 75°C and displays a decreased optimum pH value Alicyclobacillus hesperidum
D478R mutant displays a decreased optimum pH value Alicyclobacillus hesperidum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
34.2
-
 L-arabinose mutant D478A, pH 7.0, 65°C Alicyclobacillus hesperidum
41.4
-
 D-galactose mutant D478Q, pH 7.0, 65°C Alicyclobacillus hesperidum
54.7
-
 D-galactose wild-type, pH 7.0, 65°C Alicyclobacillus hesperidum
55
-
 L-arabinose mutant D478K, pH 7.0, 65°C Alicyclobacillus hesperidum
56
-
 L-arabinose mutant D478R, pH 7.0, 65°C Alicyclobacillus hesperidum
56.3
-
 L-arabinose mutant D478N, pH 7.0, 65°C Alicyclobacillus hesperidum
60.7
-
 D-galactose mutant D478R, pH 7.0, 65°C Alicyclobacillus hesperidum
66
-
 D-galactose mutant D478N, pH 7.0, 65°C Alicyclobacillus hesperidum
75.4
-
 D-galactose mutant D478K, pH 7.0, 65°C Alicyclobacillus hesperidum
83.2
-
 D-galactose mutant D478A, pH 7.0, 65°C Alicyclobacillus hesperidum
94.2
-
 L-arabinose mutant D478Q, pH 7.0, 65°C Alicyclobacillus hesperidum
105.2
-
 L-arabinose wild-type, pH 7.0, 65°C Alicyclobacillus hesperidum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 3fold increase in activity, assay in presence of 0.5 mM Alicyclobacillus hesperidum
Mn2+ 3fold increase in activity, assay in presence of 1 mM Alicyclobacillus hesperidum

Organism

Organism UniProt Comment Textmining
Alicyclobacillus hesperidum J9E728
-
-
Alicyclobacillus hesperidum URH17-3-68 J9E728
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.33
-
 substrate D-galactose, mutant D478R, pH 7.0, 65°C Alicyclobacillus hesperidum
0.52
-
 substrate D-galactose, wild-type, pH 7.0, 65°C Alicyclobacillus hesperidum
0.73
-
 substrate D-galactose, mutant D478A, pH 7.0, 65°C Alicyclobacillus hesperidum
0.75
-
 substrate D-galactose, mutant D478N, pH 7.0, 65°C Alicyclobacillus hesperidum
0.76
-
 substrate D-galactose, mutant D478K, pH 7.0, 65°C Alicyclobacillus hesperidum
0.81
-
 substrate D-galactose, mutant D478Q, pH 7.0, 65°C Alicyclobacillus hesperidum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose
-
 Alicyclobacillus hesperidum D-tagatose
-
 ?
D-galactose
-
 Alicyclobacillus hesperidum URH17-3-68 D-tagatose
-
 ?
L-arabinose
-
 Alicyclobacillus hesperidum L-ribulose
-
 ?
L-arabinose
-
 Alicyclobacillus hesperidum URH17-3-68 L-ribulose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 wild-type Alicyclobacillus hesperidum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.98
-
 D-galactose mutant D478Q, pH 7.0, 65°C Alicyclobacillus hesperidum
1.13
-
 D-galactose wild-type, pH 7.0, 65°C Alicyclobacillus hesperidum
1.26
-
 D-galactose mutant D478R, pH 7.0, 65°C Alicyclobacillus hesperidum
1.36
-
 D-galactose mutant D478K, pH 7.0, 65°C Alicyclobacillus hesperidum
1.58
-
 D-galactose mutant D478A, pH 7.0, 65°C Alicyclobacillus hesperidum
1.65
-
 D-galactose mutant D478N, pH 7.0, 65°C Alicyclobacillus hesperidum
71.8
-
 L-arabinose wild-type, pH 7.0, 65°C Alicyclobacillus hesperidum
72.9
-
 L-arabinose mutant D478R, pH 7.0, 65°C Alicyclobacillus hesperidum
85
-
 L-arabinose mutant D478A, pH 7.0, 65°C Alicyclobacillus hesperidum
99.6
-
 L-arabinose mutant D478K, pH 7.0, 65°C Alicyclobacillus hesperidum
105
-
 L-arabinose mutant D478Q, pH 7.0, 65°C Alicyclobacillus hesperidum
110
-
 L-arabinose mutant D478N, pH 7.0, 65°C Alicyclobacillus hesperidum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5 mutants D478R, D478N Alicyclobacillus hesperidum
7
-
 wild-type and mutant D478A Alicyclobacillus hesperidum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.018
-
 D-galactose mutant D478K, pH 7.0, 65°C Alicyclobacillus hesperidum
0.019
-
 D-galactose mutant D478A, pH 7.0, 65°C Alicyclobacillus hesperidum
0.02
-
 D-galactose wild-type, pH 7.0, 65°C Alicyclobacillus hesperidum
0.021
-
 D-galactose mutant D478R, pH 7.0, 65°C Alicyclobacillus hesperidum
0.024
-
 D-galactose mutant D478Q, pH 7.0, 65°C Alicyclobacillus hesperidum
0.025
-
 D-galactose mutant D478N, pH 7.0, 65°C Alicyclobacillus hesperidum
0.68
-
 L-arabinose wild-type, pH 7.0, 65°C Alicyclobacillus hesperidum
1.12
-
 L-arabinose mutant D478Q, pH 7.0, 65°C Alicyclobacillus hesperidum
1.3
-
 L-arabinose mutant D478R, pH 7.0, 65°C Alicyclobacillus hesperidum
1.81
-
 L-arabinose mutant D478K, pH 7.0, 65°C Alicyclobacillus hesperidum
1.95
-
 L-arabinose mutant D478N, pH 7.0, 65°C Alicyclobacillus hesperidum
2.48
-
 L-arabinose mutant D478A, pH 7.0, 65°C Alicyclobacillus hesperidum