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Literature summary for 5.3.1.4 extracted from

  • Zhou, X.; Wu, J.C.
    Heterologous expression and characterization of Bacillus coagulans L-arabinose isomerase (2012), World J. Microbiol. Biotechnol., 28, 2205-2212.
    View publication on PubMed
Search for more literature for 5.3.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Weizmannia coagulans

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ about 5% residual activity at 1 mM Mg2+ Weizmannia coagulans
EDTA
-
 Weizmannia coagulans
Mg2+ about 80% residual activity at 1 mM Mg2+ Weizmannia coagulans
Ni2+ about 60% residual activity at 1 mM Mg2+ Weizmannia coagulans
Zn2+ about 10% residual activity at 1 mM Mg2+ Weizmannia coagulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
106
-
 L-arabinose at pH 7.0 and 70°C Weizmannia coagulans

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ poor activator Weizmannia coagulans
Co2+ the enzyme activity is significantly increased by adding 1 mM Co2+ (2.9fold) Weizmannia coagulans
Mn2+ best activator for enzymatic activity and thermostability. The enzyme activity is significantly increased by adding 1 mM Mn2+ (4.3fold) Weizmannia coagulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53678
-
 x * 53678, calculated from amino acid sequence Weizmannia coagulans
56000
-
 x * 56000, SDS-PAGE Weizmannia coagulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arabinose Weizmannia coagulans substrate conversion of 19.4% within 1.5 h L-ribulose
-
 ?

Organism

Organism UniProt Comment Textmining
Weizmannia coagulans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-tag affinity column chromatography Weizmannia coagulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arabinose substrate conversion of 19.4% within 1.5 h Weizmannia coagulans L-ribulose
-
 ?

Subunits

Subunits Comment Organism
? x * 56000, SDS-PAGE Weizmannia coagulans
? x * 53678, calculated from amino acid sequence Weizmannia coagulans

Synonyms

Synonyms Comment Organism
L-AI
-
 Weizmannia coagulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Weizmannia coagulans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 80 about 20% activity at 30°C, about 50% activity at 40°C, about 80% activity at 60°C, 100% activity at 70°C, about 20% activity at 80°C Weizmannia coagulans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 at 50°C, the enzyme retains 100% of its initial activity after 2 h of incubation. Increasing the temperature (above 50°C) significantly reduces the enzyme stability Weizmannia coagulans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3657
-
 L-arabinose at pH 7.0 and 70°C Weizmannia coagulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Weizmannia coagulans

pH Range

pH Minimum pH Maximum Comment Organism
6 8 the enzyme retains 90% of its maximum activity at pH 6.0 and shows about 75% activity at pH 8.0. Below pH 6.0 and above pH 8.0 the activity decreases drastically Weizmannia coagulans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.575
-
 L-arabinose at pH 7.0 and 70°C Weizmannia coagulans