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Literature summary for 5.3.1.4 extracted from

  • Hong, Y.H.; Lee, D.W.; Pyun, Y.R.; Lee, S.H.
    Creation of metal-independent hyperthermophilic L-arabinose isomerase by homologous recombination (2011), J. Agric. Food Chem., 59, 12939-12947.
    View publication on PubMed

Application

Application Comment Organism
food industry hyperthermophilic L-arabinose isomerase is useful in the commercial production of D-tagatose as a low-calorie bulk sweetener Geobacillus stearothermophilus
food industry hyperthermophilic L-arabinose isomerase is useful in the commercial production of D-tagatose as a low-calorie bulk sweetener Thermotoga maritima

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Geobacillus stearothermophilus
expressed in Escherichia coli BL21(DE3) cells Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.2
-
L-arabinose in the presence of 0.8 mM Mn2+, at pH 7.5 and 60°C Thermotoga maritima
10.4
-
L-arabinose in the presence of 0.6 mM Mn2+, at pH 7.5 and 60°C Thermotoga maritima
11
-
L-arabinose in the presence of 0.2 mM Mn2+, at pH 7.5 and 60°C Thermotoga maritima
15.4
-
L-arabinose in the presence of 0.4 mM Mn2+, at pH 7.5 and 60°C Thermotoga maritima
18.6
-
L-arabinose in the presence of 0.8 mM Mn2+, at pH 7.5 and 60°C Geobacillus stearothermophilus
22.6
-
L-arabinose in the presence of 0.6 mM Mn2+, at pH 7.5 and 60°C Geobacillus stearothermophilus
25.8
-
L-arabinose in the presence of 0.1 mM Mn2+, at pH 7.5 and 60°C Thermotoga maritima
28
-
L-arabinose in the presence of 0.4 mM Mn2+, at pH 7.5 and 60°C Geobacillus stearothermophilus
39
-
L-arabinose in the presence of 0.2 mM Mn2+, at pH 7.5 and 60°C Geobacillus stearothermophilus
61
-
L-arabinose in the presence of 0.1 mM Mn2+, at pH 7.5 and 60°C Geobacillus stearothermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ the enzyme is not metal-dependent for catalytic activity but Mn2+ significantly (3fold) enhances the activity of its apo enzyme and increases thermostability Thermotoga maritima
Mn2+ the enzyme is not metal-dependent for catalytic activity but Mn2+ significantly enhances the activity of its apo enzyme and increases thermostability Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
x * 57000, SDS-PAGE Geobacillus stearothermophilus
57000
-
x * 57000, SDS-PAGE Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arabinose Geobacillus stearothermophilus
-
L-ribulose
-
?
L-arabinose Thermotoga maritima
-
L-ribulose
-
?

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-
Thermotoga maritima
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Hiprep 16/10 Q-XL column chromatography, phenyl-Sepharose column chromatography, Mono Q HR 5/5 column chromatography, and Superdex 200 gel filtration Geobacillus stearothermophilus
Hiprep 16/10 Q-XL column chromatography, phenyl-Sepharose column chromatography, Mono Q HR 5/5 column chromatography, and Superdex 200 gel filtration Thermotoga maritima

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.9
-
purified enzyme, in the absence of Mn2+, at pH 7.5 and 60°C Geobacillus stearothermophilus
2.5
-
purified enzyme, in the absence of Mn2+, at pH 7.5 and 60°C Thermotoga maritima
6.9
-
purified enzyme, in the presence of 1 mM Mn2+, at pH 7.5 and 60°C Geobacillus stearothermophilus
7.8
-
purified enzyme, in the presence of 1 mM Mn2+, at pH 7.5 and 60°C Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arabinose
-
Geobacillus stearothermophilus L-ribulose
-
?
L-arabinose
-
Thermotoga maritima L-ribulose
-
?

Subunits

Subunits Comment Organism
? x * 57000, SDS-PAGE Geobacillus stearothermophilus
? x * 57000, SDS-PAGE Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Geobacillus stearothermophilus
70
-
-
Thermotoga maritima