Application | Comment | Organism |
---|---|---|
food industry | hyperthermophilic L-arabinose isomerase is useful in the commercial production of D-tagatose as a low-calorie bulk sweetener | Geobacillus stearothermophilus |
food industry | hyperthermophilic L-arabinose isomerase is useful in the commercial production of D-tagatose as a low-calorie bulk sweetener | Thermotoga maritima |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Geobacillus stearothermophilus |
expressed in Escherichia coli BL21(DE3) cells | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.2 | - |
L-arabinose | in the presence of 0.8 mM Mn2+, at pH 7.5 and 60°C | Thermotoga maritima | |
10.4 | - |
L-arabinose | in the presence of 0.6 mM Mn2+, at pH 7.5 and 60°C | Thermotoga maritima | |
11 | - |
L-arabinose | in the presence of 0.2 mM Mn2+, at pH 7.5 and 60°C | Thermotoga maritima | |
15.4 | - |
L-arabinose | in the presence of 0.4 mM Mn2+, at pH 7.5 and 60°C | Thermotoga maritima | |
18.6 | - |
L-arabinose | in the presence of 0.8 mM Mn2+, at pH 7.5 and 60°C | Geobacillus stearothermophilus | |
22.6 | - |
L-arabinose | in the presence of 0.6 mM Mn2+, at pH 7.5 and 60°C | Geobacillus stearothermophilus | |
25.8 | - |
L-arabinose | in the presence of 0.1 mM Mn2+, at pH 7.5 and 60°C | Thermotoga maritima | |
28 | - |
L-arabinose | in the presence of 0.4 mM Mn2+, at pH 7.5 and 60°C | Geobacillus stearothermophilus | |
39 | - |
L-arabinose | in the presence of 0.2 mM Mn2+, at pH 7.5 and 60°C | Geobacillus stearothermophilus | |
61 | - |
L-arabinose | in the presence of 0.1 mM Mn2+, at pH 7.5 and 60°C | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | the enzyme is not metal-dependent for catalytic activity but Mn2+ significantly (3fold) enhances the activity of its apo enzyme and increases thermostability | Thermotoga maritima | |
Mn2+ | the enzyme is not metal-dependent for catalytic activity but Mn2+ significantly enhances the activity of its apo enzyme and increases thermostability | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
x * 57000, SDS-PAGE | Geobacillus stearothermophilus |
57000 | - |
x * 57000, SDS-PAGE | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arabinose | Geobacillus stearothermophilus | - |
L-ribulose | - |
? | |
L-arabinose | Thermotoga maritima | - |
L-ribulose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Thermotoga maritima | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Hiprep 16/10 Q-XL column chromatography, phenyl-Sepharose column chromatography, Mono Q HR 5/5 column chromatography, and Superdex 200 gel filtration | Geobacillus stearothermophilus |
Hiprep 16/10 Q-XL column chromatography, phenyl-Sepharose column chromatography, Mono Q HR 5/5 column chromatography, and Superdex 200 gel filtration | Thermotoga maritima |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.9 | - |
purified enzyme, in the absence of Mn2+, at pH 7.5 and 60°C | Geobacillus stearothermophilus |
2.5 | - |
purified enzyme, in the absence of Mn2+, at pH 7.5 and 60°C | Thermotoga maritima |
6.9 | - |
purified enzyme, in the presence of 1 mM Mn2+, at pH 7.5 and 60°C | Geobacillus stearothermophilus |
7.8 | - |
purified enzyme, in the presence of 1 mM Mn2+, at pH 7.5 and 60°C | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arabinose | - |
Geobacillus stearothermophilus | L-ribulose | - |
? | |
L-arabinose | - |
Thermotoga maritima | L-ribulose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57000, SDS-PAGE | Geobacillus stearothermophilus |
? | x * 57000, SDS-PAGE | Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Geobacillus stearothermophilus |
70 | - |
- |
Thermotoga maritima |