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Literature summary for 5.3.1.4 extracted from

  • Seo, M.J.
    Characterization of an L-arabinose isomerase from Bacillus thermoglucosidasius for D-tagatose production (2013), Biosci. Biotechnol. Biochem., 77, 385-388.
    View publication on PubMed
Search for more literature for 5.3.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Parageobacillus thermoglucosidasius

Inhibitors

Inhibitors Comment Organism Structure
Co2+ strong inhibition at 1 mM Parageobacillus thermoglucosidasius
Cu2+ complete at 1 mM Parageobacillus thermoglucosidasius
Fe2+ strong inhibition at 1 mM Parageobacillus thermoglucosidasius
Zn2+ strong inhibition at 1 mM Parageobacillus thermoglucosidasius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
175
-
 D-galactose at pH 7.0 and 40°C Parageobacillus thermoglucosidasius

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activates 2.5fold at 1 mM Parageobacillus thermoglucosidasius
additional information not influenced by Mg2+ and Ca2+ Parageobacillus thermoglucosidasius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 x * 56000, calculated from amino acid sequence Parageobacillus thermoglucosidasius
72000
-
 x * 72000, N-terminal fusion-tagged enzyme, SDS-PAGE Parageobacillus thermoglucosidasius

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arabinose Parageobacillus thermoglucosidasius
-
 L-ribulose
-
 ?
L-arabinose Parageobacillus thermoglucosidasius KCTC 1828
-
 L-ribulose
-
 ?

Organism

Organism UniProt Comment Textmining
Parageobacillus thermoglucosidasius Q6VAK6
-
-
Parageobacillus thermoglucosidasius KCTC 1828 Q6VAK6
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Parageobacillus thermoglucosidasius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose
-
 Parageobacillus thermoglucosidasius D-tagatose 45.6% conversion at 40°C ?
D-galactose
-
 Parageobacillus thermoglucosidasius KCTC 1828 D-tagatose 45.6% conversion at 40°C ?
L-arabinose
-
 Parageobacillus thermoglucosidasius L-ribulose
-
 ?
L-arabinose
-
 Parageobacillus thermoglucosidasius KCTC 1828 L-ribulose
-
 ?

Subunits

Subunits Comment Organism
? x * 56000, calculated from amino acid sequence Parageobacillus thermoglucosidasius
? x * 72000, N-terminal fusion-tagged enzyme, SDS-PAGE Parageobacillus thermoglucosidasius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Parageobacillus thermoglucosidasius

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 45 more than 80% activity between 35 and 45°C Parageobacillus thermoglucosidasius

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 60 the enzyme retains more than 80% activity after 2 h when incubated at 30, 40, 50, or 60°C Parageobacillus thermoglucosidasius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Parageobacillus thermoglucosidasius

pH Range

pH Minimum pH Maximum Comment Organism
6 9 30% activity at pH 6.0 and 9.0, 100% activity at pH 7.0, 90% activity at pH 8.0 Parageobacillus thermoglucosidasius

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.047
-
 D-galactose at pH 7.0 and 40°C Parageobacillus thermoglucosidasius