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Literature summary for 5.3.1.4 extracted from

  • Cao, T.P.; Choi, J.M.; Lee, S.J.; Lee, Y.J.; Lee, S.K.; Jun, Y.; Lee, D.W.; Lee, S.H.
    Crystallization and preliminary X-ray crystallographic analysis of L-arabinose isomerase from thermophilic Geobacillus kaustophilus (2014), Acta Crystallogr. Sect. F, 70, 108-112.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Geobacillus kaustophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1 M imidazole hydrochloric acid pH 8.0, 1 M sodium citrate tribasic Geobacillus kaustophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required for activity Geobacillus kaustophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56200
-
 6 * 56200, calculated from amino acid sequence Geobacillus kaustophilus
58000
-
 6 * 58000, His-tagged enzyme, SDS-PAGE Geobacillus kaustophilus
321000
-
 gel filtration Geobacillus kaustophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arabinose Geobacillus kaustophilus
-
 L-ribulose
-
 ?

Organism

Organism UniProt Comment Textmining
Geobacillus kaustophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography and Superdex 200 gel filtration Geobacillus kaustophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.84
-
 in the absence of Mn2+, at pH 7.5 and 60°C Geobacillus kaustophilus
4.03
-
 in the presence of Mn2+, at pH 7.5 and 60°C Geobacillus kaustophilus
4.57
-
 purified enzyme, at pH 7.5 and 60°C Geobacillus kaustophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arabinose
-
 Geobacillus kaustophilus L-ribulose
-
 ?

Subunits

Subunits Comment Organism
homohexamer 6 * 56200, calculated from amino acid sequence Geobacillus kaustophilus
homohexamer 6 * 58000, His-tagged enzyme, SDS-PAGE Geobacillus kaustophilus

pI Value

Organism Comment pI Value Maximum pI Value
Geobacillus kaustophilus calculated from amino acid sequence
-
 5.4