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Literature summary for 5.3.1.4 extracted from

  • Cheng, L.; Mu, W.; Jiang, B.
    Thermostable L-arabinose isomerase from Bacillus stearothermophilus IAM 11001 for D-tagatose production: gene cloning, purification and characterisation (2010), J. Sci. Food Agric., 90, 1327-1333.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Geobacillus stearothermophilus

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 6% residual activity at 1 mM Geobacillus stearothermophilus
Ca2+ complete inhibition at 1 mM Geobacillus stearothermophilus
Co2+ 90% residual activity at 1 mM Geobacillus stearothermophilus
Cu2+ 53% residual activity at 1 mM Geobacillus stearothermophilus
EDTA 22% residual activity at 1 mM Geobacillus stearothermophilus
Mg2+ 90% residual activity at 1 mM Geobacillus stearothermophilus
Zn2+ 61% residual activity at 1 mM Geobacillus stearothermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 106% relative activity at 1 mM Geobacillus stearothermophilus
Mn2+ 404% relative activity at 1 mM, the enzyme has an absolute requirement for the divalent metal ion Mn2+ for both catalytic activity and thermostability Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
x * 56000, enzyme with C-terminal His-tag, SDS-PAGE Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-
Geobacillus stearothermophilus IAM 11001
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose column chromatography, gel filtration Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose the bioconversion yield of D-galactose to D-tagatose by the purified enzyme after 12 h at 65°C reaches 36% Geobacillus stearothermophilus D-tagatose
-
?
D-galactose the bioconversion yield of D-galactose to D-tagatose by the purified enzyme after 12 h at 65°C reaches 36% Geobacillus stearothermophilus IAM 11001 D-tagatose
-
?
L-arabinose
-
Geobacillus stearothermophilus L-ribulose
-
?
L-arabinose
-
Geobacillus stearothermophilus IAM 11001 L-ribulose
-
?

Subunits

Subunits Comment Organism
? x * 56000, enzyme with C-terminal His-tag, SDS-PAGE Geobacillus stearothermophilus

Synonyms

Synonyms Comment Organism
L-AI
-
Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Geobacillus stearothermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 70
-
Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Geobacillus stearothermophilus

pH Range

pH Minimum pH Maximum Comment Organism
5 8.5 the enzyme exhibits 82% and 65% of its maximum activity at pH 6.0 and 5.0 respectively and maintains more than 80% activity at pH values ranging from 6.0 to 8.5 Geobacillus stearothermophilus