Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Geobacillus stearothermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 6% residual activity at 1 mM | Geobacillus stearothermophilus | |
Ca2+ | complete inhibition at 1 mM | Geobacillus stearothermophilus | |
Co2+ | 90% residual activity at 1 mM | Geobacillus stearothermophilus | |
Cu2+ | 53% residual activity at 1 mM | Geobacillus stearothermophilus | |
EDTA | 22% residual activity at 1 mM | Geobacillus stearothermophilus | |
Mg2+ | 90% residual activity at 1 mM | Geobacillus stearothermophilus | |
Zn2+ | 61% residual activity at 1 mM | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 106% relative activity at 1 mM | Geobacillus stearothermophilus | |
Mn2+ | 404% relative activity at 1 mM, the enzyme has an absolute requirement for the divalent metal ion Mn2+ for both catalytic activity and thermostability | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
x * 56000, enzyme with C-terminal His-tag, SDS-PAGE | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Geobacillus stearothermophilus IAM 11001 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-Sepharose column chromatography, gel filtration | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose | the bioconversion yield of D-galactose to D-tagatose by the purified enzyme after 12 h at 65°C reaches 36% | Geobacillus stearothermophilus | D-tagatose | - |
? | |
D-galactose | the bioconversion yield of D-galactose to D-tagatose by the purified enzyme after 12 h at 65°C reaches 36% | Geobacillus stearothermophilus IAM 11001 | D-tagatose | - |
? | |
L-arabinose | - |
Geobacillus stearothermophilus | L-ribulose | - |
? | |
L-arabinose | - |
Geobacillus stearothermophilus IAM 11001 | L-ribulose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56000, enzyme with C-terminal His-tag, SDS-PAGE | Geobacillus stearothermophilus |
Synonyms | Comment | Organism |
---|---|---|
L-AI | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Geobacillus stearothermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 70 | - |
Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Geobacillus stearothermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | the enzyme exhibits 82% and 65% of its maximum activity at pH 6.0 and 5.0 respectively and maintains more than 80% activity at pH values ranging from 6.0 to 8.5 | Geobacillus stearothermophilus |