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Literature summary for 5.3.1.4 extracted from

  • Kim, H.; Uhm, T.; Kim, S.; Kim, P.
    Escherichia coli arabinose isomerase and Staphylococcus aureus tagatose-6-phosphate isomerase: Which is a better template for directed evolution of non-natural substrate isomerization? (2010), J. Microbiol. Biotechnol., 20, 1018-1021.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli ER2566 cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
G270D the mutation causes a decrease in activity of more than 20% compared to the wild type enzyme Escherichia coli
L282M the mutation causes a decrease in activity of more than 20% compared to the wild type enzyme Escherichia coli
R25C the mutation causes a decrease in activity of more than 20% compared to the wild type enzyme Escherichia coli
T451P the enzyme shows 31% increased activity compared to the wild type enzyme Escherichia coli
Y496C the mutation causes a decrease in activity of more than 20% compared to the wild type enzyme Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
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