Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Borate | the catalytic efficiency (kcat/Km) for D-galactose with borate is twice as much as that without borate | Anoxybacillus flavithermus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Anoxybacillus flavithermus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | about 85% residual activity at 1 mM | Anoxybacillus flavithermus | |
Cu2+ | about 25% residual activity at 1 mM | Anoxybacillus flavithermus | |
Fe3+ | about 35% residual activity at 1 mM | Anoxybacillus flavithermus | |
Li+ | about 90% residual activity at 1 mM | Anoxybacillus flavithermus | |
Mg2+ | about 90% residual activity at 1 mM | Anoxybacillus flavithermus | |
Mn2+ | about 70% residual activity at 1 mM | Anoxybacillus flavithermus | |
Na+ | about 90% residual activity at 1 mM | Anoxybacillus flavithermus | |
Zn2+ | about 65% residual activity at 1 mM | Anoxybacillus flavithermus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
25.19 | - |
D-galactose | without borate, in 50 mM glycine-NaOH buffer (pH 9.5), at 95°C | Anoxybacillus flavithermus | |
26.81 | - |
D-galactose | with 10 mM borate, in 50 mM glycine-NaOH buffer (pH 9.5), at 95°C | Anoxybacillus flavithermus | |
78.5 | - |
L-arabinose | in 50 mM glycine-NaOH buffer (pH 9.5), at 95°C | Anoxybacillus flavithermus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ni2+ | about 135% activity at 1 mM | Anoxybacillus flavithermus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55876 | - |
x * 55876, calculated from amino acid sequence | Anoxybacillus flavithermus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anoxybacillus flavithermus | F6KRI7 | - |
- |
Anoxybacillus flavithermus TC-06 | F6KRI7 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Anoxybacillus flavithermus |
Storage Stability | Organism |
---|---|
4°C, glycine-NaOH buffer (pH 9.5), 60 days, no loss of activity | Anoxybacillus flavithermus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose | the enzyme has high preference for D-galactose (100% activity) | Anoxybacillus flavithermus | D-tagatose | - |
? | |
D-galactose | the enzyme has high preference for D-galactose (100% activity) | Anoxybacillus flavithermus TC-06 | D-tagatose | - |
? | |
D-Xylose | 32.3% activity compared to D-galactose | Anoxybacillus flavithermus | ? | - |
? | |
D-Xylose | 32.3% activity compared to D-galactose | Anoxybacillus flavithermus TC-06 | ? | - |
? | |
L-arabinose | 40% activity compared to D-galactose | Anoxybacillus flavithermus | L-ribulose | - |
? | |
L-arabinose | 40% activity compared to D-galactose | Anoxybacillus flavithermus TC-06 | L-ribulose | - |
? | |
additional information | D-glucose and D-allose do not serve as substrate | Anoxybacillus flavithermus | ? | - |
? | |
additional information | D-glucose and D-allose do not serve as substrate | Anoxybacillus flavithermus TC-06 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55876, calculated from amino acid sequence | Anoxybacillus flavithermus |
Synonyms | Comment | Organism |
---|---|---|
L-AI | - |
Anoxybacillus flavithermus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
- |
Anoxybacillus flavithermus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | 85 | the enzyme is not active below 75°C, the relative activity is less than 50% below 85°C | Anoxybacillus flavithermus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
L-arabinose | in 50 mM glycine-NaOH buffer (pH 9.5), at 95°C | Anoxybacillus flavithermus | |
2.2 | - |
D-galactose | without borate, in 50 mM glycine-NaOH buffer (pH 9.5), at 95°C | Anoxybacillus flavithermus | |
78.5 | - |
D-galactose | with 10 mM borate, in 50 mM glycine-NaOH buffer (pH 9.5), at 95°C | Anoxybacillus flavithermus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | 10.5 | - |
Anoxybacillus flavithermus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9.5 | the enzyme is not active below pH 7.0, the relative activity is less than 50% below pH 9.5 | Anoxybacillus flavithermus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Anoxybacillus flavithermus | calculated from amino acid sequence | - |
5.1 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
L-arabinose | in 50 mM glycine-NaOH buffer (pH 9.5), at 95°C | Anoxybacillus flavithermus | |
0.09 | - |
D-galactose | without borate, in 50 mM glycine-NaOH buffer (pH 9.5), at 95°C | Anoxybacillus flavithermus | |
0.16 | - |
D-galactose | with 10 mM borate, in 50 mM glycine-NaOH buffer (pH 9.5), at 95°C | Anoxybacillus flavithermus |