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Literature summary for 5.3.1.4 extracted from

  • Oh, D.K.; Oh, H.J.; Kim, H.J.; Cheon, J.; Kim, P.
    Modification of optimal pH in L-arabinose isomerase from Geobacillus stearothermophilus for D-galactose isomerization (2006), J. Mol. Catal. B, 43, 108-112.
No PubMed abstract available
Search for more literature for 5.3.1.4

Application

Application Comment Organism
food industry production of D-tagatose as a low-calorie sugar-substituting sweetener lower pH is preferable for industrial production Geobacillus stearothermophilus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Geobacillus stearothermophilus

Protein Variants

Protein Variants Comment Organism
A408V site directed mutagenesis Geobacillus stearothermophilus
A408V/K475N site directed mutagenesis Geobacillus stearothermophilus
A475N site directed mutagenesis Geobacillus stearothermophilus
D228N site directed mutagenesis Geobacillus stearothermophilus
D228N/D384G/T393S/N428K/K475N site directed mutagenesis, GSAI 152 Geobacillus stearothermophilus
D384G site directed mutagenesis Geobacillus stearothermophilus
G408V site directed mutagenesis Geobacillus stearothermophilus
K475N site directed mutagenesis Geobacillus stearothermophilus
L408V site directed mutagenesis Geobacillus stearothermophilus
N428K site directed mutagenesis Geobacillus stearothermophilus
Q408V site directed mutagenesis Geobacillus stearothermophilus
Q475N site directed mutagenesis Geobacillus stearothermophilus
R408V site directed mutagenesis Geobacillus stearothermophilus
R475N site directed mutagenesis Geobacillus stearothermophilus
T393S site directed mutagenesis Geobacillus stearothermophilus
V322M site directed mutagenesis Geobacillus stearothermophilus
V322M/T393S/A408V site directed mutagenesis, GSAI 153 Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus Q9S467 Geobacillus stearothermophilus
-

Purification (Commentary)

Purification (Comment) Organism
of the recombinant wild type and mutant enzymes Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.004
-
 D228N mutant, D-galactose as substrate Geobacillus stearothermophilus
0.029
-
 R408V mutant, D-galactose as substrate Geobacillus stearothermophilus
0.043
-
 wild type, D-galactose as substrate Geobacillus stearothermophilus
0.05
-
 L408V mutant, D-galactose as substrate Geobacillus stearothermophilus
0.056
-
 D384G mutant, D-galactose as substrate Geobacillus stearothermophilus
0.06
-
 Q408V mutant, at pH 7.5, D-galactose as substrate Geobacillus stearothermophilus
0.06
-
 R408V mutant, at pH 7.5, D-galactose as substrate Geobacillus stearothermophilus
0.062
-
 Q408V mutant, D-galactose as substrate Geobacillus stearothermophilus
0.11
-
 T393S mutant, D-galactose as substrate Geobacillus stearothermophilus
0.26
-
 N428K mutant, D-galactose as substrate Geobacillus stearothermophilus
0.27
-
 V322M mutant, D-galactose as substrate Geobacillus stearothermophilus
0.37
-
 G408V mutant, D-galactose as substrate Geobacillus stearothermophilus
0.37
-
 Q475N mutant, D-galactose as substrate Geobacillus stearothermophilus
0.38
-
 R475N mutant, D-galactose as substrate Geobacillus stearothermophilus
0.39
-
 K475N mutant found in GSAI152, D-galactose as substrate Geobacillus stearothermophilus
0.39
-
 K475N mutant, D-galactose as substrate Geobacillus stearothermophilus
0.48
-
 A408V mutant, D-galactose as substrate Geobacillus stearothermophilus
0.48
-
 A475N mutant found in GSAI153, D-galactose as substrate Geobacillus stearothermophilus
0.49
-
 GSAI1 152, D-galactose as substrate Geobacillus stearothermophilus
0.59
-
 A408V/K475N mutant found in GSAI152 or GASI 153w D-galactose as substrate Geobacillus stearothermophilus
0.74
-
 GSAI1 153, D-galactose as substrate Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose
-
 Geobacillus stearothermophilus D-tagatose
-
 ?
L-arabinose
-
 Geobacillus stearothermophilus L-ribulose
-
 ?

Synonyms

Synonyms Comment Organism
D-galactose isomerase
-
 Geobacillus stearothermophilus
GSAI Geobacillus stearothermophilus L-arabinose isomease Geobacillus stearothermophilus
GSAI 152 five mutations Geobacillus stearothermophilus
GSAI 153 three mutations Geobacillus stearothermophilus
L-arabinose aldose-ketose-isomerase
-
 Geobacillus stearothermophilus
L-arabinose isomerase
-
 Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 Q408V mutant enzyme with higher isomerization activites than the wild type enzyme Geobacillus stearothermophilus
7.5
-
 R408V mutant enzyme with higher isomerization activites than the wild type enzyme Geobacillus stearothermophilus
8.5
-
 wild type enzyme Geobacillus stearothermophilus