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Literature summary for 5.3.1.4 extracted from
- Probing the essential catalytic residues and substrate affinity in the thermoactive Bacillus stearothermophilus US100 L-arabinose isomerase by site-directed mutagenesis (2007), J. Bacteriol., 189, 3556-3563.
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | production of D-tagatose | Geobacillus stearothermophilus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Geobacillus stearothermophilus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D308A | site directed mutagenesis | Geobacillus stearothermophilus |
| E306A | site directed mutagenesis, no activity | Geobacillus stearothermophilus |
| E331A | site directed mutagenesis, no activity | Geobacillus stearothermophilus |
| E351A | site directed mutagenesis | Geobacillus stearothermophilus |
| F279Q | site directed mutagenesis | Geobacillus stearothermophilus |
| F329A | site directed mutagenesis | Geobacillus stearothermophilus |
| H348A | site directed mutagenesis, no activity | Geobacillus stearothermophilus |
| H446A | site directed mutagenesis | Geobacillus stearothermophilus |
| H447A | site directed mutagenesis, no activity | Geobacillus stearothermophilus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 28.42 | - |
L-arabinose | wild type | Geobacillus stearothermophilus |  |
| 29.3 | - |
L-arabinose | D308A mutant | Geobacillus stearothermophilus | |
| 30.1 | - |
L-arabinose | E351A mutant | Geobacillus stearothermophilus | |
| 30.4 | - |
L-arabinose | H446A mutant | Geobacillus stearothermophilus | |
| 30.6 | - |
L-arabinose | F329A mutant | Geobacillus stearothermophilus | |
| 79.7 | - |
L-arabinose | F279Q mutant | Geobacillus stearothermophilus | |
| 173.6 | - |
L-fucose | F279Q mutant | Geobacillus stearothermophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | is closely bound to the protein even after treatment with EDTA | Geobacillus stearothermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
SDS-PAGE | Geobacillus stearothermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | Q9S467 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| of the recombinant mutant proteins | Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 18 | - |
fucose as substrate, F279Q mutant | Geobacillus stearothermophilus |
| 21 | - |
D308A mutant | Geobacillus stearothermophilus |
| 27 | - |
E351A mutant | Geobacillus stearothermophilus |
| 53 | - |
F329A mutant | Geobacillus stearothermophilus |
| 79 | - |
H446A mutant | Geobacillus stearothermophilus |
| 184 | - |
wild type | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactose | - |
Geobacillus stearothermophilus | D-tagatose | - |
? | |
| L-arabinose | - |
Geobacillus stearothermophilus | L-ribulose | - |
? | |
| L-Fucose | only F279 mutant | Geobacillus stearothermophilus | L-Fuculose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-galactose isomerase | - |
Geobacillus stearothermophilus |
| L-AI US100 | - |
Geobacillus stearothermophilus |
| L-arabinose aldose-ketose-isomerase | - |
Geobacillus stearothermophilus |
| L-arabinose isomerase | - |
Geobacillus stearothermophilus |
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