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Literature summary for 5.3.1.4 extracted from
- Characterization of a mutated Geobacillus stearothermophilus L-arabinose isomerase that increases the production rate of D-tagatose (2006), J. Appl. Microbiol., 101, 213-221.
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | production of D-tagatose as a low-calorie sugar-substituting sweetener, the D-tagatose yield from the mutated enzyme is higher than from the wild type | Geobacillus stearothermophilus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli of wild type and gali 153 mutant enzyme | Geobacillus stearothermophilus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| M322V/S393T/V408A | error prone PCR mutagenesis using gali 152 as template, gali 153 with changes in 3 amino acids revealed a higher activity than gali 152 | Geobacillus stearothermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Geobacillus stearothermophilus |  |
| Dulcitol | weak inhibitor | Geobacillus stearothermophilus | |
| erythritol | weak inhibitor | Geobacillus stearothermophilus | |
| L-arabitol | strong inhibitory | Geobacillus stearothermophilus | |
| ribitol | strong inhibitory | Geobacillus stearothermophilus | |
| xylitol | weak inhibitor | Geobacillus stearothermophilus | |
| Zn2+ | - |
Geobacillus stearothermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 67 | - |
L-arabinose | wild type enzyme | Geobacillus stearothermophilus | |
| 100 | - |
L-arabinose | mutated enzyme | Geobacillus stearothermophilus | |
| 145 | - |
D-galactose | wild type enzyme | Geobacillus stearothermophilus | |
| 578 | - |
D-galactose | mutated enzyme | Geobacillus stearothermophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | activating | Geobacillus stearothermophilus | |
| Ca2+ | activating | Geobacillus stearothermophilus | |
| Co2+ | activating, highest activity of the mutated enzyme at 1.0 mM | Geobacillus stearothermophilus | |
| Cu2+ | inhibitory | Geobacillus stearothermophilus | |
| Fe2+ | activating | Geobacillus stearothermophilus | |
| Mg2+ | activating | Geobacillus stearothermophilus | |
| Mn2+ | activating, highest activity of the wild type enzyme at 1.0 mM | Geobacillus stearothermophilus | |
| Mo2+ | activating | Geobacillus stearothermophilus | |
| Zn2+ | inhibitory | Geobacillus stearothermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
SDS-PAGE | Geobacillus stearothermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | Q9S467 | - |
- |
| Geobacillus stearothermophilus KCCM12265 | Q9S467 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| of the recombinant wild type and mutant enzymes | Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.3 | - |
of the recombinant wild type enzyme after purification | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactose | - |
Geobacillus stearothermophilus | D-tagatose | - |
? | |
| D-galactose | - |
Geobacillus stearothermophilus KCCM12265 | D-tagatose | - |
? | |
| L-arabinose | other aldoses like D-fucose, D-ribose, D-allose, D-mannose and D-xylose are poor substrates for the wild type and the mutated enzymes | Geobacillus stearothermophilus | L-ribulose | - |
? | |
| L-arabinose | other aldoses like D-fucose, D-ribose, D-allose, D-mannose and D-xylose are poor substrates for the wild type and the mutated enzymes | Geobacillus stearothermophilus KCCM12265 | L-ribulose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-galactose isomerase | - |
Geobacillus stearothermophilus |
| gali 152 | mutated L-arabinose isomerase from pL152 gene | Geobacillus stearothermophilus |
| gali 153 | mutated L-arabinose isomerase from pL153 gene | Geobacillus stearothermophilus |
| L-arabinose aldose-ketose-isomerase | - |
Geobacillus stearothermophilus |
| L-arabinose isomerase | - |
Geobacillus stearothermophilus |
| pL 151 | - |
Geobacillus stearothermophilus |
| pL 152 | - |
Geobacillus stearothermophilus |
| pL 153 | - |
Geobacillus stearothermophilus |
| pL151 | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
wild type enzyme | Geobacillus stearothermophilus |
| 65 | - |
mutated enzyme gali 153 | Geobacillus stearothermophilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | 75 | - |
Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
wild type and mutated enzyme | Geobacillus stearothermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | - |
Geobacillus stearothermophilus |
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