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Literature summary for 5.3.1.4 extracted from
- Production of D-tagatose at high temperatures using immobilized Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana (2007), Biotechnol. Lett., 29, 569-574.
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | production of D-tagatose as a low-calorie sugar-substituting sweetener, reactor was run for 50 days | Thermotoga neapolitana |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli cells. that are immobilized in calcium alginate beads | Thermotoga neapolitana |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 1mM for the free enzyme and for the immobilized enzyme in Escherichis coli cells | Thermotoga neapolitana |  |
| Mn2+ | 1mM for the free enzyme, 10 mM for the immobilized enzyme in Escherichis coli cells | Thermotoga neapolitana | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga neapolitana | - |
Escherichia coli cells expressing L-arabinose isomerase | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactose | - |
Thermotoga neapolitana | D-tagatose | - |
? | |
| L-arabinose | - |
Thermotoga neapolitana | L-ribulose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-galactose isomerase | - |
Thermotoga neapolitana |
| L-arabinose aldose-ketose-isomerase | - |
Thermotoga neapolitana |
| L-arabinose isomerase | - |
Thermotoga neapolitana |
| TNAI | Thermotoga neapolitana L-arabinose isomerase | Thermotoga neapolitana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
free enzyme | Thermotoga neapolitana |
| 90 | - |
immobilized enzyme in Escherichis coli cells | Thermotoga neapolitana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
free enzyme | Thermotoga neapolitana |
| 7 | - |
immobilized enzyme in Escherichis coli cells | Thermotoga neapolitana |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.5 | immobilized enzyme in Escherichis coli cells, for 7 days at 25°C | Thermotoga neapolitana |
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