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Literature summary for 5.3.1.4 extracted from

  • Oh, H.J.; Kim, H.J.; Oh, D.K.
    Increase in D-tagatose production rate by site-directed mutagenesis of L-arabinose isomerase from Geobacillus thermodenitrificans (2006), Biotechnol. Lett., 28, 145-149.
    View publication on PubMed
Search for more literature for 5.3.1.4

Application

Application Comment Organism
food industry production of D-tagatose Geobacillus thermodenitrificans

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Geobacillus thermodenitrificans

Protein Variants

Protein Variants Comment Organism
C450S site directed mutagenesis Geobacillus thermodenitrificans
C450S/N475K site directed mutagenesis, 20% higher tagatose conversion than the wild type enzyme Geobacillus thermodenitrificans
G384D site directed mutagenesis Geobacillus thermodenitrificans
K320R site directed mutagenesis Geobacillus thermodenitrificans
K320R/N475K site directed mutagenesis Geobacillus thermodenitrificans
K428N site directed mutagenesis Geobacillus thermodenitrificans
K428N/N475K site directed mutagenesis Geobacillus thermodenitrificans
M322V site directed mutagenesis Geobacillus thermodenitrificans
N228D site directed mutagenesis Geobacillus thermodenitrificans
N475K site directed mutagenesis Geobacillus thermodenitrificans
N475Q site directed mutagenesis Geobacillus thermodenitrificans
N475R site directed mutagenesis Geobacillus thermodenitrificans
S393T site directed mutagenesis Geobacillus thermodenitrificans
V408A site directed mutagenesis Geobacillus thermodenitrificans
V408A/N475K site directed mutagenesis Geobacillus thermodenitrificans
W164G site directed mutagenesis Geobacillus thermodenitrificans
Y164F site directed mutagenesis Geobacillus thermodenitrificans
Y164G site directed mutagenesis Geobacillus thermodenitrificans
Y164H site directed mutagenesis Geobacillus thermodenitrificans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
15
-
 L-arabinose M322V mutant Geobacillus thermodenitrificans
36
-
 L-arabinose W164G mutant Geobacillus thermodenitrificans
36
-
 L-arabinose Y164G mutant Geobacillus thermodenitrificans
45
-
 D-galactose N475K mutant Geobacillus thermodenitrificans
51
-
 L-arabinose C450S mutant Geobacillus thermodenitrificans
56
-
 L-arabinose K320R mutant Geobacillus thermodenitrificans
58
-
 L-arabinose K320R/N475K mutant Geobacillus thermodenitrificans
59
-
 L-arabinose K428N mutant Geobacillus thermodenitrificans
66
-
 L-arabinose V408A /N475K mutant Geobacillus thermodenitrificans
67
-
 D-galactose C450S mutant Geobacillus thermodenitrificans
69
-
 L-arabinose N475K mutant Geobacillus thermodenitrificans
71
-
 L-arabinose C450S /N475K mutant Geobacillus thermodenitrificans
75
-
 D-galactose C450S /N475K mutant Geobacillus thermodenitrificans
76
-
 L-arabinose V408A mutant Geobacillus thermodenitrificans
82
-
 L-arabinose N475R mutant Geobacillus thermodenitrificans
90
-
 D-galactose K428N mutant Geobacillus thermodenitrificans
90
-
 L-arabinose Y164H mutant Geobacillus thermodenitrificans
94
-
 L-arabinose N475Q mutant Geobacillus thermodenitrificans
97
-
 L-arabinose wild type Geobacillus thermodenitrificans
97
-
 D-galactose V408A mutant Geobacillus thermodenitrificans
97
-
 L-arabinose Y164F mutant Geobacillus thermodenitrificans
101
-
 D-galactose Y164F mutant Geobacillus thermodenitrificans
103
-
 D-galactose K320R/N475K mutant Geobacillus thermodenitrificans
105
-
 D-galactose N475Q mutant Geobacillus thermodenitrificans
123
-
 L-arabinose N228D mutant Geobacillus thermodenitrificans
130
-
 D-galactose Y164H mutant Geobacillus thermodenitrificans
144
-
 D-galactose wild type Geobacillus thermodenitrificans
148
-
 D-galactose K320R mutant Geobacillus thermodenitrificans
149
-
 D-galactose K428N /N475K mutant Geobacillus thermodenitrificans
161
-
 D-galactose N228D mutant Geobacillus thermodenitrificans
163
-
 L-arabinose G384D mutant Geobacillus thermodenitrificans
168
-
 L-arabinose K428N /N475K mutant Geobacillus thermodenitrificans
173
-
 D-galactose N475R mutant Geobacillus thermodenitrificans
188
-
 L-arabinose S393T mutant Geobacillus thermodenitrificans
201
-
 D-galactose S393T mutant Geobacillus thermodenitrificans
257
-
 D-galactose V408A /N475K mutant Geobacillus thermodenitrificans
280
-
 D-galactose M322V mutant Geobacillus thermodenitrificans
713
-
 D-galactose G384D mutant Geobacillus thermodenitrificans
1105
-
 D-galactose W164G mutant Geobacillus thermodenitrificans
1105
-
 D-galactose Y164G mutant Geobacillus thermodenitrificans

Organism

Organism UniProt Comment Textmining
Geobacillus thermodenitrificans Q6W9D4
-
-

Purification (Commentary)

Purification (Comment) Organism
of the recombinant mutant proteins by HisTrap HP chromatography Geobacillus thermodenitrificans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.14
-
 wild type, D-galactose as substrate Geobacillus thermodenitrificans
4.82
-
 wild type, L-arabinose as substrate Geobacillus thermodenitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose
-
 Geobacillus thermodenitrificans D-tagatose
-
 ?
L-arabinose
-
 Geobacillus thermodenitrificans L-ribulose
-
 ?

Synonyms

Synonyms Comment Organism
D-galactose isomerase
-
 Geobacillus thermodenitrificans
L-arabinose aldose-ketose-isomerase
-
 Geobacillus thermodenitrificans
L-arabinose isomerase
-
 Geobacillus thermodenitrificans