Application | Comment | Organism |
---|---|---|
nutrition | the product D-tagatose is currently introduced as a low-calorie bulk sweetener | Thermoanaerobacter mathranii |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermotoga maritima |
expression in Escherichia coli | Thermoanaerobacter mathranii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
80 | - |
L-arabinose | - |
Thermoanaerobacter mathranii | |
120 | - |
D-galactose | - |
Thermoanaerobacter mathranii | |
145 | - |
D-fucose | - |
Thermoanaerobacter mathranii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | addition of CoSO4 reactivates the enzyme to 45% after inactivation with EDTA | Thermoanaerobacter mathranii | |
Co2+ | preferred cofactor | Thermotoga maritima | |
Fe2+ | addition of FeSO4 reactivates the enzyme to 76% after inactivation with EDTA | Thermoanaerobacter mathranii | |
Mn2+ | addition of MnCl2 reactivates the enzyme to 100% after inactivation with EDTA | Thermoanaerobacter mathranii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
monomer, SDS-PAGE | Thermoanaerobacter mathranii |
52820 | - |
monomer, calculated from amino acid sequence | Thermoanaerobacter mathranii |
55000 | - |
monomer, SDS-PAGE | Thermotoga maritima |
212000 | - |
gel filtration | Thermotoga maritima |
220000 | - |
gel filtration | Thermoanaerobacter mathranii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose | Thermotoga maritima | - |
D-tagatose | - |
? | |
D-galactose | Thermoanaerobacter mathranii | - |
D-tagatose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoanaerobacter mathranii | Q70G56 | araA | - |
Thermotoga maritima | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme purified from Escherichia coli | Thermotoga maritima |
recombinant enzyme purified from Escherichia coli | Thermoanaerobacter mathranii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.055 | - |
recovered activity after immobilization of the enzyme | Thermoanaerobacter mathranii |
4 | - |
after purification to homogeneity | Thermotoga maritima |
4 | - |
after purification to homogeneity | Thermoanaerobacter mathranii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Fucose | - |
Thermoanaerobacter mathranii | ? | - |
? | |
D-galactose | - |
Thermotoga maritima | D-tagatose | - |
? | |
D-galactose | - |
Thermoanaerobacter mathranii | D-tagatose | - |
? | |
L-arabinose | - |
Thermoanaerobacter mathranii | L-ribulose | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 52000, SDS-PAGE, gel filtration | Thermoanaerobacter mathranii |
tetramer | 4 * 55000, SDS-PAGE, gel filtration | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
L-arabinose isomerase | - |
Thermotoga maritima |
L-arabinose isomerase | - |
Thermoanaerobacter mathranii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Thermoanaerobacter mathranii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Thermoanaerobacter mathranii |