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Literature summary for 5.3.1.4 extracted from

  • Jorgensen, F.; Hansen, O.C.; Stougaard, P.
    Enzymatic conversion of D-galactose to D-tagatose: heterologous expression and characterisation of a thermostable L-arabinose isomerase from Thermoanaerobacter mathranii (2004), Appl. Microbiol. Biotechnol., 64, 816-822.
    View publication on PubMed

Application

Application Comment Organism
nutrition the product D-tagatose is currently introduced as a low-calorie bulk sweetener Thermoanaerobacter mathranii

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermotoga maritima
expression in Escherichia coli Thermoanaerobacter mathranii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
80
-
L-arabinose
-
Thermoanaerobacter mathranii
120
-
D-galactose
-
Thermoanaerobacter mathranii
145
-
D-fucose
-
Thermoanaerobacter mathranii

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ addition of CoSO4 reactivates the enzyme to 45% after inactivation with EDTA Thermoanaerobacter mathranii
Co2+ preferred cofactor Thermotoga maritima
Fe2+ addition of FeSO4 reactivates the enzyme to 76% after inactivation with EDTA Thermoanaerobacter mathranii
Mn2+ addition of MnCl2 reactivates the enzyme to 100% after inactivation with EDTA Thermoanaerobacter mathranii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
monomer, SDS-PAGE Thermoanaerobacter mathranii
52820
-
monomer, calculated from amino acid sequence Thermoanaerobacter mathranii
55000
-
monomer, SDS-PAGE Thermotoga maritima
212000
-
gel filtration Thermotoga maritima
220000
-
gel filtration Thermoanaerobacter mathranii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-galactose Thermotoga maritima
-
D-tagatose
-
?
D-galactose Thermoanaerobacter mathranii
-
D-tagatose
-
?

Organism

Organism UniProt Comment Textmining
Thermoanaerobacter mathranii Q70G56 araA
-
Thermotoga maritima
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme purified from Escherichia coli Thermotoga maritima
recombinant enzyme purified from Escherichia coli Thermoanaerobacter mathranii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.055
-
recovered activity after immobilization of the enzyme Thermoanaerobacter mathranii
4
-
after purification to homogeneity Thermotoga maritima
4
-
after purification to homogeneity Thermoanaerobacter mathranii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Fucose
-
Thermoanaerobacter mathranii ?
-
?
D-galactose
-
Thermotoga maritima D-tagatose
-
?
D-galactose
-
Thermoanaerobacter mathranii D-tagatose
-
?
L-arabinose
-
Thermoanaerobacter mathranii L-ribulose
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 52000, SDS-PAGE, gel filtration Thermoanaerobacter mathranii
tetramer 4 * 55000, SDS-PAGE, gel filtration Thermotoga maritima

Synonyms

Synonyms Comment Organism
L-arabinose isomerase
-
Thermotoga maritima
L-arabinose isomerase
-
Thermoanaerobacter mathranii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Thermoanaerobacter mathranii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Thermoanaerobacter mathranii