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Literature summary for 5.3.1.4 extracted from

  • Lee, S.J.; Lee, D.W.; Choe, E.A.; Hong, Y.H.; Kim, S.B.; Kim, B.C.; Pyun, Y.R.
    Characterization of a thermoacidophilic L-arabinose isomerase from Alicyclobacillus acidocaldarius: role of Lys-269 in pH optimum (2005), Appl. Environ. Microbiol., 71, 7888-7896.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Halalkalibacterium halodurans
expression in Escherichia coli Alicyclobacillus acidocaldarius

Protein Variants

Protein Variants Comment Organism
E268K pH optimum shifts from pH 8 to 7 Halalkalibacterium halodurans
K269E pH optimum shifts from pH 6 to 7 Alicyclobacillus acidocaldarius

Inhibitors

Inhibitors Comment Organism Structure
EDTA 1 mM inhibits activity completely Alicyclobacillus acidocaldarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
38.2
-
 L-arabinose wild-type at pH 6 and 50°C Halalkalibacterium halodurans
39.7
-
 L-arabinose wild-type at pH 7 and 50°C Halalkalibacterium halodurans
43.7
-
 L-arabinose mutant E268K at pH 7 and 50°C Halalkalibacterium halodurans
43.8
-
 L-arabinose wild-type at pH 8 and 50°C Halalkalibacterium halodurans
46
-
 L-arabinose mutant E268K at pH 8 and 50°C Halalkalibacterium halodurans
46.2
-
 L-arabinose wild-type at pH 7 and 65°C Alicyclobacillus acidocaldarius
47.5
-
 L-arabinose wild-type at pH 8 and 65°C Alicyclobacillus acidocaldarius
48
-
 L-arabinose wild-type at pH 6 and 65°C Alicyclobacillus acidocaldarius
52.5
-
 L-arabinose mutant K269E at pH 8 and 65°C Alicyclobacillus acidocaldarius
53.8
-
 L-arabinose mutant K269E at pH 7 and 65°C Alicyclobacillus acidocaldarius
56.1
-
 L-arabinose mutant E268K at pH 6 and 50°C Halalkalibacterium halodurans
57.9
-
 L-arabinose mutant K269E at pH 6 and 65°C Alicyclobacillus acidocaldarius
129
-
 D-galactose wild-type at pH 6 and 65°C Alicyclobacillus acidocaldarius

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 1 mM required for activity Alicyclobacillus acidocaldarius
Mg2+ 1 mM required for activity Alicyclobacillus acidocaldarius
Mn2+ 1 mM required for activity Alicyclobacillus acidocaldarius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56040
-
 monomer, calculated from amino acid sequence Alicyclobacillus acidocaldarius
57000
-
 monomer, SDS-PAGE Alicyclobacillus acidocaldarius
224000
-
 gel filtration Alicyclobacillus acidocaldarius

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-galactose Halalkalibacterium halodurans
-
 D-tagatose
-
 ?
D-galactose Alicyclobacillus acidocaldarius
-
 D-tagatose
-
 ?
L-arabinose Halalkalibacterium halodurans
-
 L-ribulose
-
 ?
L-arabinose Alicyclobacillus acidocaldarius
-
 L-ribulose
-
 ?

Organism

Organism UniProt Comment Textmining
Alicyclobacillus acidocaldarius Q2VMT2
-
-
Halalkalibacterium halodurans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Halalkalibacterium halodurans
recombinant enzyme is purified to homogeneity by heat treatment, ion-exchange chromatography, and gel filtration Alicyclobacillus acidocaldarius

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.5
-
 wild-type with D-galactose as substrate at pH 6 and 65°C Alicyclobacillus acidocaldarius
17
-
 wild-type with L-arabinose as substrate at pH 6 and 50°C Halalkalibacterium halodurans
21
-
 mutant K269E with L-arabinose as substrate at pH 6 and 65°C Alicyclobacillus acidocaldarius
25.3
-
 wild-type with L-arabinose as substrate at pH 7 and 50°C Halalkalibacterium halodurans
25.8
-
 mutant E268K with L-arabinose as substrate at pH 6 and 50°C Halalkalibacterium halodurans
26.8
-
 wild-type with L-arabinose as substrate at pH 8 and 65°C Alicyclobacillus acidocaldarius
27.6
-
 mutant K269E with L-arabinose as substrate at pH 8 and 65°C Alicyclobacillus acidocaldarius
30.6
-
 wild-type with L-arabinose as substrate at pH 7 and 65°C Alicyclobacillus acidocaldarius
31.7
-
 mutant E268K with L-arabinose as substrate at pH 8 and 50°C Halalkalibacterium halodurans
33.2
-
 wild-type with L-arabinose as substrate at pH 8 and 50°C Halalkalibacterium halodurans
35.3
-
 mutant E268K with L-arabinose as substrate at pH 7 and 50°C Halalkalibacterium halodurans
35.5
-
 wild-type with L-arabinose as substrate at pH 6 and 65°C Alicyclobacillus acidocaldarius
39.9
-
 mutant K269E with L-arabinose as substrate at pH 7 and 65°C Alicyclobacillus acidocaldarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose
-
 Halalkalibacterium halodurans D-tagatose
-
 ?
D-galactose
-
 Alicyclobacillus acidocaldarius D-tagatose
-
 ?
L-arabinose
-
 Halalkalibacterium halodurans L-ribulose
-
 ?
L-arabinose
-
 Alicyclobacillus acidocaldarius L-ribulose
-
 ?

Subunits

Subunits Comment Organism
tetramer alpha4, 4 * 57000, SDS-PAGE, gel filtration Alicyclobacillus acidocaldarius

Synonyms

Synonyms Comment Organism
AI
-
 Halalkalibacterium halodurans
AI
-
 Alicyclobacillus acidocaldarius
L-arabinose isomerase
-
 Halalkalibacterium halodurans
L-arabinose isomerase
-
 Alicyclobacillus acidocaldarius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Halalkalibacterium halodurans
65
-
-
 Alicyclobacillus acidocaldarius

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
15.88
-
 L-arabinose wild-type at pH 6 and 50°C Halalkalibacterium halodurans
19.62
-
 L-arabinose mutant K269E at pH 6 and 65°C Alicyclobacillus acidocaldarius
23.65
-
 L-arabinose wild-type at pH 7 and 50°C Halalkalibacterium halodurans
24.11
-
 L-arabinose mutant E268K at pH 6 and 50°C Halalkalibacterium halodurans
24.97
-
 L-arabinose wild-type at pH 8 and 65°C Alicyclobacillus acidocaldarius
25.8
-
 L-arabinose mutant K269E at pH 8 and 65°C Alicyclobacillus acidocaldarius
28.55
-
 L-arabinose wild-type at pH 7 and 65°C Alicyclobacillus acidocaldarius
29.63
-
 L-arabinose mutant E268K at pH 8 and 50°C Halalkalibacterium halodurans
31.03
-
 L-arabinose wild-type at pH 8 and 50°C Halalkalibacterium halodurans
32.93
-
 L-arabinose mutant E268K at pH 7 and 50°C Halalkalibacterium halodurans
33.15
-
 L-arabinose wild-type at pH 6 and 65°C Alicyclobacillus acidocaldarius
37.2
-
 L-arabinose mutant K269E at pH 7 and 65°C Alicyclobacillus acidocaldarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5 wild-type Alicyclobacillus acidocaldarius
7
-
 mutant E268K Halalkalibacterium halodurans
7
-
 mutant K269E Alicyclobacillus acidocaldarius
7.5 8 wild-type Halalkalibacterium halodurans

pH Range

pH Minimum pH Maximum Comment Organism
5.2 8 more than 50% of the maximal activity Alicyclobacillus acidocaldarius

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 half-life: about 10 h Alicyclobacillus acidocaldarius
6
-
 AI retains its original activity even after 48 h incubation Alicyclobacillus acidocaldarius

pI Value

Organism Comment pI Value Maximum pI Value
Alicyclobacillus acidocaldarius isoelectric focusing
-
 5
Alicyclobacillus acidocaldarius calculated
-
 5.51