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Literature summary for 5.3.1.4 extracted from
- Cloning, expression and characterization of L-arabinose isomerase from Thermotoga neapolitana: bioconversion of D-galactose to D-tagatose using the enzyme (2002), FEMS Microbiol. Lett., 212, 121-126.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Thermotoga neapolitana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 116 | - |
L-arabinose | pH 7.0, 85°C | Thermotoga neapolitana |  |
| 250 | - |
D-galactose | pH 7.0, 85°C | Thermotoga neapolitana | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | divalent cation, maximal activity in presence of both Mn2+ and Co2+ | Thermotoga neapolitana | |
| Mn2+ | divalent cation, maximal activity in presence of both Mn2+ and Co2+ | Thermotoga neapolitana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56677 | - |
x * 56677, calculation from nucleotide sequence | Thermotoga neapolitana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga neapolitana | - |
5068 | - |
| Thermotoga neapolitana 5068 | - |
5068 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Thermotoga neapolitana |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Thermotoga neapolitana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactose | the conversion equilibrium shifts to more ketone sugar from aldose sugar as the isomerization reaction temperature raises. 68% conversion of D-galactose to D-tagatose at 80°C | Thermotoga neapolitana | D-tagatose | - |
r | |
| D-galactose | the conversion equilibrium shifts to more ketone sugar from aldose sugar as the isomerization reaction temperature raises. 68% conversion of D-galactose to D-tagatose at 80°C | Thermotoga neapolitana 5068 | D-tagatose | - |
r | |
| L-arabinose | - |
Thermotoga neapolitana | L-ribulose | - |
? | |
| L-arabinose | - |
Thermotoga neapolitana 5068 | L-ribulose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 56677, calculation from nucleotide sequence | Thermotoga neapolitana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
L-arabinose and D-galactose isomerization | Thermotoga neapolitana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
2 h, in absence of metal ions more than 50% loss of activity, in presence of Co2+ and Mn2+ the enzyme retains 80% of its original activity | Thermotoga neapolitana |
| 90 | - |
half-life without metal ions: 70 min, half-life with 1 mM CoCl2 and 1 mM MnCl2: 120 min | Thermotoga neapolitana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Thermotoga neapolitana |
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