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Literature summary for 5.3.1.4 extracted from

  • Lee, D.W.; Jang, H.J.; Choe, E.A.; Kim, B.C.; Lee, S.J.; Kim, S.B.; Hong, Y.H.; Pyun, Y.R.
    Characterization of a thermostable L-arabinose (D-galactose) isomerase from the hyperthermophilic eubacterium Thermotoga maritima (2004), Appl. Environ. Microbiol., 70, 1397-1404.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.1.4

Application

Application Comment Organism
nutrition the enzyme may be a good model for analysis of metal-mediated thermostabilization and for industrial application in the production of D-tagatose as a novel sweetener Thermotoga maritima

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli as a fusion protein containing a C-terminal hexahistidine sequence Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
31
-
 L-arabinose pH 7.5, 90°C Thermotoga maritima
60
-
 D-galactose pH 7.5, 90°C Thermotoga maritima

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ poor activator Thermotoga maritima
Co2+ Mn2+ or Co2+ required, about 1 mM Co2+ restores activity of dialyzed enzyme Thermotoga maritima
Fe2+ poor activator Thermotoga maritima
Mg2+ poor activator Thermotoga maritima
Mn2+ Mn2+ or Co2+ required, about 5 mM Mn2+ restores activity of dialyzed enzyme Thermotoga maritima
Ni2+ poor activator Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56658
-
 4 * 56658, calculation from nucleotide sequence Thermotoga maritima
57000
-
 4 * 57000, SDS-PAGE Thermotoga maritima
230000
-
 gel filtration Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose 56% conversion after 6 h at 80°C Thermotoga maritima D-tagatose
-
 ?
L-arabinose 70% conversion to L-ribulose in 6 h at 80°C Thermotoga maritima L-ribulose
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 57000, SDS-PAGE Thermotoga maritima
tetramer 4 * 56658, calculation from nucleotide sequence Thermotoga maritima

Synonyms

Synonyms Comment Organism
AI
-
 Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
 in absence of metal ions Thermotoga maritima
90
-
 in presence of metal ions Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 more thermostable in presence of Mn2+ and/or Co2+ than in absence Thermotoga maritima
80
-
 240 min, incubation in presence of Mn2+ or Co2+ causes little loss of activity, apoenzyme is very unstable in absence of metal ions Thermotoga maritima
90
-
 half-life in presence of 5 mM Mn2+: 185 min Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 90°C, in presence of 1 mM Co2+ and 5 mM Mn2+ Thermotoga maritima

pI Value

Organism Comment pI Value Maximum pI Value
Thermotoga maritima isoelectric focusing, pH-gradient: pH 3.0-10.0
-
 5.7