Cloned (Comment) | Organism |
---|---|
into the vector pGEM-T Easy, and subsequently into the vector pET15b for expression in Escherichia coli ER2566 cells | Caldicellulosiruptor saccharolyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
61 | - |
D-altrose | - |
Caldicellulosiruptor saccharolyticus | |
152 | - |
D-arabinose | - |
Caldicellulosiruptor saccharolyticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Caldicellulosiruptor saccharolyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
determined by SDS-PAGE | Caldicellulosiruptor saccharolyticus |
68110 | - |
theoretical | Caldicellulosiruptor saccharolyticus |
204000 | - |
homotrimer, determined by gel filtration | Caldicellulosiruptor saccharolyticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-altrose | Caldicellulosiruptor saccharolyticus | - |
D-psicose | - |
? | |
D-arabinose | Caldicellulosiruptor saccharolyticus | - |
D-ribulose | - |
? | |
L-galactose | Caldicellulosiruptor saccharolyticus | - |
L-tagatose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caldicellulosiruptor saccharolyticus | A4XJ56 | - |
- |
Purification (Comment) | Organism |
---|---|
on a His-Trap HP column | Caldicellulosiruptor saccharolyticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2 | - |
substrate L-galactose | Caldicellulosiruptor saccharolyticus |
15 | - |
substrate D-altrose | Caldicellulosiruptor saccharolyticus |
62 | - |
substrate D-arabinose | Caldicellulosiruptor saccharolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-altrose | - |
Caldicellulosiruptor saccharolyticus | D-psicose | - |
? | |
D-arabinose | - |
Caldicellulosiruptor saccharolyticus | D-ribulose | - |
? | |
L-galactose | - |
Caldicellulosiruptor saccharolyticus | L-tagatose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 3 * 68000 Da | Caldicellulosiruptor saccharolyticus |
Synonyms | Comment | Organism |
---|---|---|
D-Arabinose isomerase | L-fucose isomerase, bifunctional enzyme | Caldicellulosiruptor saccharolyticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0287 | - |
D-altrose | - |
Caldicellulosiruptor saccharolyticus | |
0.1823 | - |
D-arabinose | - |
Caldicellulosiruptor saccharolyticus |