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Literature summary for 5.3.1.29 extracted from

  • Nakamura, A.; Fujihashi, M.; Aono, R.; Sato, T.; Nishiba, Y.; Yoshida, S.; Yano, A.; Atomi, H.; Imanaka, T.; Miki, K.
    Dynamic, ligand-dependent conformational change triggers reaction of ribose-1,5-bisphosphate isomerase from Thermococcus kodakarensis KOD1 (2012), J. Biol. Chem., 287, 20784-20796.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermococcus kodakarensis

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method at 20°C. Crystal structure of unliganded Tk-R15Pi is solved by means of the single-wavelength anomalous dispersion method using SeMet-substituted enzyme and refined at 2.5 A resolution Thermococcus kodakarensis

Protein Variants

Protein Variants Comment Organism
C133A inactive mutant enzyme Thermococcus kodakarensis
C133S inactive mutant enzyme Thermococcus kodakarensis
D202N inactive mutant enzyme Thermococcus kodakarensis
R227R mutant enzyme exhibits a decrease in molecular size of the enzyme and significantly decreases the enzymatic activity Thermococcus kodakarensis

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis Q5JFM9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermococcus kodakarensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
29.3
-
pH 8.0, 85°C, wild-type enzyme Thermococcus kodakarensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-ribose 1,5-bisphosphate the enzyme is specific for the alpha-anomer of D-ribose 1,5-bisphosphate Thermococcus kodakarensis D-ribulose 1,5-bisphosphate
-
?

Subunits

Subunits Comment Organism
hexamer
-
Thermococcus kodakarensis

Synonyms

Synonyms Comment Organism
R15Pi
-
Thermococcus kodakarensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
assay at Thermococcus kodakarensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Thermococcus kodakarensis