BRENDA - Enzyme Database show
show all sequences of 5.3.1.29

Enzymatic characterization of AMP phosphorylase and ribose-1,5-bisphosphate isomerase functioning in an archaeal AMP metabolic pathway

Aono, R.; Sato, T.; Yano, A.; Yoshida, S.; Nishitani, Y.; Miki, K.; Imanaka, T.; Atomi, H.; J. Bacteriol. 194, 6847-6855 (2012)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.6
-
alpha-D-ribose 1,5-bisphosphate
pH 8.3, 85°C
Thermococcus kodakarensis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
alpha-D-ribose 1,5-bisphosphate
Thermococcus kodakarensis
the archaeal enzyme is involved in AMP metabolism and CO2 fixation through type III RubisCO enzymes
D-ribulose 1,5-bisphosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermococcus kodakarensis
Q5JFM9
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-D-ribose 1,5-bisphosphate
the archaeal enzyme is involved in AMP metabolism and CO2 fixation through type III RubisCO enzymes
725279
Thermococcus kodakarensis
D-ribulose 1,5-bisphosphate
-
-
-
?
alpha-D-ribose 1,5-bisphosphate
the enzyme is specific for the alpha-anomer of D-ribose 1,5-bisphosphate and does not recognize other sugar compounds (ribose 5-phosphate, phosphoribosylpyrophosphate, fructose 1,6-bisphosphate, fructose 6-phosphate, glucose, pyruvate or 3-phosphoglycerate)
725279
Thermococcus kodakarensis
D-ribulose 1,5-bisphosphate
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
85
-
assay at
Thermococcus kodakarensis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
29.2
-
alpha-D-ribose 1,5-bisphosphate
pH 8.3, 85°C
Thermococcus kodakarensis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.3
-
assay at
Thermococcus kodakarensis
Cofactor
Cofactor
Commentary
Organism
Structure
AMP
activity is extremely low with the substrate alpha-D-ribose 1,5-bisphosphate alone but is more than 40fold activated in the presence of AMP
Thermococcus kodakarensis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
AMP
activity is extremely low with the substrate alpha-D-ribose 1,5-bisphosphate alone but is more than 40fold activated in the presence of AMP
Thermococcus kodakarensis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.6
-
alpha-D-ribose 1,5-bisphosphate
pH 8.3, 85°C
Thermococcus kodakarensis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
alpha-D-ribose 1,5-bisphosphate
Thermococcus kodakarensis
the archaeal enzyme is involved in AMP metabolism and CO2 fixation through type III RubisCO enzymes
D-ribulose 1,5-bisphosphate
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-D-ribose 1,5-bisphosphate
the archaeal enzyme is involved in AMP metabolism and CO2 fixation through type III RubisCO enzymes
725279
Thermococcus kodakarensis
D-ribulose 1,5-bisphosphate
-
-
-
?
alpha-D-ribose 1,5-bisphosphate
the enzyme is specific for the alpha-anomer of D-ribose 1,5-bisphosphate and does not recognize other sugar compounds (ribose 5-phosphate, phosphoribosylpyrophosphate, fructose 1,6-bisphosphate, fructose 6-phosphate, glucose, pyruvate or 3-phosphoglycerate)
725279
Thermococcus kodakarensis
D-ribulose 1,5-bisphosphate
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
85
-
assay at
Thermococcus kodakarensis
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
29.2
-
alpha-D-ribose 1,5-bisphosphate
pH 8.3, 85°C
Thermococcus kodakarensis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.3
-
assay at
Thermococcus kodakarensis
Expression
Organism
Commentary
Expression
Thermococcus kodakarensis
increased protein levels of the ribose 1,5-bisphosphate isomerase in the cells cultivated with nucleosides
up
Expression (protein specific)
Organism
Commentary
Expression
Thermococcus kodakarensis
increased protein levels of the ribose 1,5-bisphosphate isomerase in the cells cultivated with nucleosides
up
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
48.7
-
alpha-D-ribose 1,5-bisphosphate
pH 8.3, 85°C
Thermococcus kodakarensis
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
48.7
-
alpha-D-ribose 1,5-bisphosphate
pH 8.3, 85°C
Thermococcus kodakarensis
Other publictions for EC 5.3.1.29
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746485
Gogoi
A presumed homologue of the r ...
Pyrococcus horikoshii, Pyrococcus horikoshii DSM 12428, Pyrococcus horikoshii OT3
Sci. Rep.
8
1891
2018
4
-
2
2
2
1
-
-
-
-
-
-
-
7
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
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-
-
4
-
2
-
2
2
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
733939
Gogoi
In silico analysis suggests th ...
Pyrococcus horikoshii, Pyrococcus horikoshii DSM 12428
Gene
575
118-126
2016
-
-
-
1
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
725279
Aono
Enzymatic characterization of ...
Thermococcus kodakarensis
J. Bacteriol.
194
6847-6855
2012
-
-
-
-
-
-
-
1
-
-
-
1
-
3
-
-
-
-
-
-
-
-
2
-
1
-
-
1
1
-
-
1
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-
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1
-
-
-
-
-
-
1
-
-
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1
-
-
-
-
-
-
-
-
2
-
1
-
-
1
1
-
-
-
1
-
-
1
1
1
727903
Nakamura
Dynamic, ligand-dependent conf ...
Thermococcus kodakarensis
J. Biol. Chem.
287
20784-20796
2012
-
-
1
1
4
-
-
-
-
-
-
-
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3
-
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1
-
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1
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1
1
1
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-
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1
-
-
-
-
-
-
-
-
1
-
1
4
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
726487
Sato
Archaeal type III RuBisCOs fun ...
Thermococcus kodakarensis
Science
315
1003-1006
2007
-
-
-
-
-
-
-
-
-
-
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1
-
1
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-
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-
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1
-
2
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-
-
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-
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-
-
-
-
-
-
-
-
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1
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-
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-
1
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2
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